Cytotoxic Activity of Baccharis trimera (Less.) DC. Essential Oil in Tumor Cell Lines and its Role in Associated Death Mechanisms

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dc.contributor.authorMoro, Isabela Jacob [UNESP]
dc.contributor.authorCarvalho, Flavio Alexandre [UNESP]
dc.contributor.authorMoreira, Thais Fernanda [UNESP]
dc.contributor.authorSouza, Felipe de Oliveira [UNESP]
dc.contributor.authorda Silva, Alexander Alves [UNESP]
dc.contributor.authorPoliti, Flávio [UNESP]
dc.contributor.authorSoares, Christiane Pienna [UNESP]
dc.contributor.authorDos Santos, André Gonzaga [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2023-07-29T13:53:26Z
dc.date.available2023-07-29T13:53:26Z
dc.date.issued2023-01-01
dc.description.abstractThis study evaluated the potential of Baccharis trimera essential oil (EO) and its components in cancer therapy through the application of cytotoxicity and cell death assays. Using gas chromatography analysis, the major components of the aerial parts of the essential oil were identified as (E)-caryophyllene (18.9%), bicyclogermacrene (15.6%), and germacrene D (10.5%). Baccharis trimera essential oil (5.8 μg/mL) and α-humulene (7.8 μg/mL) presented strong cytotoxic activity, while (E)-caryophyllene (11.5 μg/mL) and caryophyllene oxide (> 100.0 μg/mL) showed moderate and low activities, respectively, against MCF-7 cell lines. Against HepG2 cell lines, B. trimera essential oil (10.4 μg/mL), α-humulene (17.1 μg/mL), and caryophyllene oxide (19.4 μg/mL) exhibited moderate activity, while (E)-caryophyllene (52.3 μg/mL) displayed low activity against HepG2 cell lines. The selectivity index values of EO (MCF-7 and HepG2), α-humulene and (E)-caryophyllene (MCF-7), and caryophyllene oxide (HepG2) were found between 1.1 and 2.8, compared with MCF-10A cells. The annexin-V and Hoechst / propidium iodide assays performed with essential oil, (E)-caryophyllene, α-humulene, and caryophyllene oxide showed apoptosis and necrosis mechanisms for all cell lines. Based on these findings, B. trimera essential oil and its components can be considered as potential therapeutic agents against cancer.en
dc.description.affiliationSão Paulo State University (UNESP) School of Pharmaceutical Sciences Department of Drugs and Medicines, Rod. Araraquara-Jaú Km 01 s/n, São Paulo
dc.description.affiliationSão Paulo State University (UNESP) School of Pharmaceutical Sciences Department of Clinical Analysis, Rod. Araraquara-Jaú Km 01 s/n, São Paulo
dc.description.affiliationSão Paulo State University (UNESP) Chemistry Institute Department of Organic Chemistry, Rue Prof. Francisco Degni n. 55,, São Paulo
dc.description.affiliationUnespSão Paulo State University (UNESP) School of Pharmaceutical Sciences Department of Drugs and Medicines, Rod. Araraquara-Jaú Km 01 s/n, São Paulo
dc.description.affiliationUnespSão Paulo State University (UNESP) School of Pharmaceutical Sciences Department of Clinical Analysis, Rod. Araraquara-Jaú Km 01 s/n, São Paulo
dc.description.affiliationUnespSão Paulo State University (UNESP) Chemistry Institute Department of Organic Chemistry, Rue Prof. Francisco Degni n. 55,, São Paulo
dc.format.extent21-26
dc.identifierhttp://dx.doi.org/10.17807/orbital.v15i1.17021
dc.identifier.citationOrbital, v. 15, n. 1, p. 21-26, 2023.
dc.identifier.doi10.17807/orbital.v15i1.17021
dc.identifier.issn1984-6428
dc.identifier.scopus2-s2.0-85156275230
dc.identifier.urihttp://hdl.handle.net/11449/248776
dc.language.isoeng
dc.relation.ispartofOrbital
dc.sourceScopus
dc.subjectBaccharis trimera
dc.subjectcancer
dc.subjectcytotoxic
dc.subjectessential oil
dc.titleCytotoxic Activity of Baccharis trimera (Less.) DC. Essential Oil in Tumor Cell Lines and its Role in Associated Death Mechanismsen
dc.typeArtigo
unesp.departmentAnálises Clínicas - FCFpt
unesp.departmentFármacos e Medicamentos - FCFpt

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Araraquara - FCF - Faculdade de Ciências Farmacêuticas
Artigos - Química Orgânica - IQ