Salmonella enterica serovar typhimurium colonizing the lumen of the chicken intestine grows slowly and upregulates a unique set of virulence and metabolism genes

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dc.contributor.authorHarvey, P. C.
dc.contributor.authorWatson, M.
dc.contributor.authorHulme, S.
dc.contributor.authorJones, M. A.
dc.contributor.authorLovell, M.
dc.contributor.authorBerchieri, Jr. [UNESP]
dc.contributor.authorYoung, J.
dc.contributor.authorBumstead, N.
dc.contributor.authorBarrow, P.
dc.contributor.institutionCompton Laboratory
dc.contributor.institutionRoslin Biocentre
dc.contributor.institutionUniversity of Nottingham
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:26:01Z
dc.date.available2014-05-27T11:26:01Z
dc.date.issued2011-10-01
dc.description.abstractThe pattern of global gene expression in Salmonella enterica serovar Typhimurium bacteria harvested from the chicken intestinal lumen (cecum) was compared with that of a late-log-phase LB broth culture using a whole-genome microarray. Levels of transcription, translation, and cell division in vivo were lower than those in vitro. S. Typhimurium appeared to be using carbon sources, such as propionate, 1,2-propanediol, and ethanolamine, in addition to melibiose and ascorbate, the latter possibly transformed to D-xylulose. Amino acid starvation appeared to be a factor during colonization. Bacteria in the lumen were non- or weakly motile and nonchemotactic but showed upregulation of a number of fimbrial and Salmonella pathogenicity island 3 (SPI-3) and 5 genes, suggesting a close physical association with the host during colonization. S. Typhimurium bacteria harvested from the cecal mucosa showed an expression profile similar to that of bacteria from the intestinal lumen, except that levels of transcription, translation, and cell division were higher and glucose may also have been used as a carbon source. © 2011, American Society for Microbiology.en
dc.description.affiliationInstitute for Animal Health Compton Laboratory, Compton, Newbury, Berkshire RG20 7NN
dc.description.affiliationThe Roslin Institute The University of Edinburgh Roslin Biocentre, Roslin, Midlothian EH25 9PS, Scotland
dc.description.affiliationSchool of Veterinary Medicine and Science University of Nottingham, Sutton Bonington, Loughborough, Leicestershire LE12 5RD
dc.description.affiliationFaculdade de Ciencas Agrárias e Veterinárias Universidade Estadual Paulista, 14870-000 Jaboticabal, São Paulo
dc.description.affiliationUnespFaculdade de Ciencas Agrárias e Veterinárias Universidade Estadual Paulista, 14870-000 Jaboticabal, São Paulo
dc.format.extent4105-4121
dc.identifierhttp://dx.doi.org/10.1128/IAI.01390-10
dc.identifier.citationInfection and Immunity, v. 79, n. 10, p. 4105-4121, 2011.
dc.identifier.doi10.1128/IAI.01390-10
dc.identifier.file2-s2.0-80855136572.pdf
dc.identifier.issn0019-9567
dc.identifier.issn1098-5522
dc.identifier.scopus2-s2.0-80855136572
dc.identifier.urihttp://hdl.handle.net/11449/72708
dc.language.isoeng
dc.relation.ispartofInfection and Immunity
dc.relation.ispartofjcr3.256
dc.relation.ispartofsjr1,954
dc.relation.ispartofsjr1,954
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectarginine
dc.subjectascorbic acid
dc.subjectcarbon
dc.subjectcitrate synthase
dc.subjectDNA binding protein
dc.subjectDNA directed DNA polymerase gamma
dc.subjectethanolamine
dc.subjectflagellin
dc.subjectglucose
dc.subjecthost factor 1
dc.subjectmelibiose
dc.subjectpropionic acid
dc.subjectpropylene glycol
dc.subjectputrescine derivative
dc.subjectthreonine dehydratase
dc.subjectxylulose
dc.subjectamino acid metabolism
dc.subjectamino acid transport
dc.subjectanimal tissue
dc.subjectbacterial colonization
dc.subjectcarbon source
dc.subjectcecum
dc.subjectcell division
dc.subjectchromosome replication
dc.subjectcontrolled study
dc.subjectcross reaction
dc.subjectDNA replication
dc.subjectdown regulation
dc.subjectenergy yield
dc.subjectflagellum
dc.subjectgene control
dc.subjectgene expression
dc.subjectgenetic transcription
dc.subjectin vitro study
dc.subjectin vivo study
dc.subjectintestine
dc.subjectintestine mucosa
dc.subjectnonhuman
dc.subjectpathogenicity
dc.subjectpriority journal
dc.subjectSalmonella enterica
dc.subjectSalmonella typhimurium
dc.subjectupregulation
dc.subjectvirulence
dc.subjectAnimals
dc.subjectBacterial Proteins
dc.subjectCecum
dc.subjectChickens
dc.subjectGene Expression Profiling
dc.subjectGene Expression Regulation, Bacterial
dc.subjectGenome, Bacterial
dc.subjectMice
dc.subjectMice, Inbred BALB C
dc.subjectOligonucleotide Array Sequence Analysis
dc.subjectPoultry Diseases
dc.subjectSalmonella Infections, Animal
dc.subjectSpecific Pathogen-Free Organisms
dc.subjectUp-Regulation
dc.subjectVirulence
dc.subjectVirulence Factors
dc.titleSalmonella enterica serovar typhimurium colonizing the lumen of the chicken intestine grows slowly and upregulates a unique set of virulence and metabolism genesen
dc.typeArtigo
dcterms.licensehttp://journals.asm.org/site/misc/ASM_Author_Statement.xhtml
unesp.author.lattes3508096260678286[6]
unesp.author.orcid0000-0003-2522-6500[6]

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