Challenges and Opportunities in Clinical Diagnostic Routine of Envenomation Using Blood Plasma Proteomics

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dc.contributor.authorCavalcante, Joeliton dos Santos [UNESP]
dc.contributor.authorde Almeida, Denis Emanuel Garcia [UNESP]
dc.contributor.authorMoraes, Micael Saggion [UNESP]
dc.contributor.authorSantos, Sophia Ribeiro [UNESP]
dc.contributor.authorPincinato, Pedro Moriel [UNESP]
dc.contributor.authorRiciopo, Pedro Marques [UNESP]
dc.contributor.authorde Oliveira, Laís Lacerda B.
dc.contributor.authorMonteiro, Wuelton Marcelo
dc.contributor.authorFerreira-Junior, Rui Seabra [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionFederal University of Ceará (UFC)
dc.contributor.institutionAmazonas State University
dc.date.accessioned2023-07-29T13:48:15Z
dc.date.available2023-07-29T13:48:15Z
dc.date.issued2023-03-01
dc.description.abstractSpecific and sensitive tools for the diagnosis and monitoring of accidents by venomous animals are urgently needed. Several diagnostic and monitoring assays have been developed; however, they have not yet reached the clinic. This has resulted in late diagnoses, which represents one of the main causes of progression from mild to severe disease. Human blood is a protein-rich biological fluid that is routinely collected in hospital settings for diagnostic purposes, which can translate research progress from the laboratory to the clinic. Although it is a limited view, blood plasma proteins provide information about the clinical picture of envenomation. Proteome disturbances in response to envenomation by venomous animals have been identified, allowing mass spectrometry (MS)-based plasma proteomics to emerge as a tool in a range of clinical diagnostics and disease management that can be applied to cases of venomous animal envenomation. Here, we provide a review of the state of the art on routine laboratory diagnoses of envenomation by snakes, scorpions, bees, and spiders, as well as a review of the diagnostic methods and the challenges encountered. We present the state of the art on clinical proteomics as the standardization of procedures to be performed within and between research laboratories, favoring a more excellent peptide coverage of candidate proteins for biomarkers. Therefore, the selection of a sample type and method of preparation should be very specific and based on the discovery of biomarkers in specific approaches. However, the sample collection protocol (e.g., collection tube type) and the processing procedure of the sample (e.g., clotting temperature, time allowed for clotting, and anticoagulant used) are equally important to eliminate any bias.en
dc.description.affiliationGraduate Program in Tropical Diseases Botucatu Medical School (FMB) São Paulo State University (UNESP—Universidade Estadual Paulista), São Paulo
dc.description.affiliationDepartment of Bioprocess and Biotechnology School of Agriculture Agronomic Sciences São Paulo State University (UNESP—Universidade Estadual Paulista), São Paulo
dc.description.affiliationGraduate Program in Translational Medicine Drug Research and Development Center (NPDM) Federal University of Ceará (UFC), Ceará
dc.description.affiliationGraduate Program in Tropical Medicine Department of Research at Fundação de Medicina Tropical Dr. Heitor Vieira Dourado Amazonas State University, Amazonas
dc.description.affiliationCenter for the Study of Venoms and Venomous Animals (CEVAP) São Paulo State University (UNESP—Universidade Estadual Paulista), São Paulo
dc.description.affiliationUnespGraduate Program in Tropical Diseases Botucatu Medical School (FMB) São Paulo State University (UNESP—Universidade Estadual Paulista), São Paulo
dc.description.affiliationUnespDepartment of Bioprocess and Biotechnology School of Agriculture Agronomic Sciences São Paulo State University (UNESP—Universidade Estadual Paulista), São Paulo
dc.description.affiliationUnespCenter for the Study of Venoms and Venomous Animals (CEVAP) São Paulo State University (UNESP—Universidade Estadual Paulista), São Paulo
dc.identifierhttp://dx.doi.org/10.3390/toxins15030180
dc.identifier.citationToxins, v. 15, n. 3, 2023.
dc.identifier.doi10.3390/toxins15030180
dc.identifier.issn2072-6651
dc.identifier.scopus2-s2.0-85151114012
dc.identifier.urihttp://hdl.handle.net/11449/248590
dc.language.isoeng
dc.relation.ispartofToxins
dc.sourceScopus
dc.subjectbiomarkers
dc.subjectblood plasma
dc.subjectclinical proteomics
dc.subjecthuman envenoming
dc.titleChallenges and Opportunities in Clinical Diagnostic Routine of Envenomation Using Blood Plasma Proteomicsen
dc.typeResenha
unesp.author.orcid0000-0001-5712-9137[1]
unesp.author.orcid0000-0002-0848-1940[8]
unesp.author.orcid0000-0001-6952-0512[9]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt
unesp.departmentDoenças Tropicais e Diagnósticos por Imagem - FMBpt

Arquivos

Coleções

Botucatu - FMB - Faculdade de Medicina
Botucatu - CEVAP - Centro de Estudos de Venenos e Animais Peçonhentos