In vivo embryo development in bitches inseminated laparoscopically after ovulation time estimated based on a single progesterone determination

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dc.contributor.authorAlves, Aracelle Elisane
dc.contributor.authorMotheo, Tathiana Ferguson
dc.contributor.authorApparicio, Maricy Ferreira [UNESP]
dc.contributor.authorMostachio, Giuliano Queiroz
dc.contributor.authordos Santos, Ricarda Maria
dc.contributor.authorVicente, Wilter Ricardo Russiano [UNESP]
dc.contributor.authorLuvoni, Gaia Cecilia
dc.contributor.institutionUniversidade Federal de Uberlândia (UFU)
dc.contributor.institutionUniversidade de Cuiabá
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionCentro Universitário de Rio Preto
dc.contributor.institutionUniversità Degli Studi di Milano
dc.date.accessioned2023-07-29T13:46:24Z
dc.date.available2023-07-29T13:46:24Z
dc.date.issued2023-01-01
dc.description.abstractLogistic and economical limitations are often the causes of dog owners not accurately monitoring the estrous cycle and the optimal insemination time. The aim of this study was to evaluate in vivo early embryonic development in bitches, after the analysis of sequential vaginal cytologies associated to single progesterone measurement and single laparoscopic insemination with high quality semen (fresh and with high spermatozoa concentration) or low-quality semen (frozen/thawed and with low spermatozoa concentration) at 48 h post-ovulation time predicted on a single progesterone measurement. Ten bitches were inseminated with 250 x 106 fresh spermatozoa (80% motility), and ten with 80 x 106 frozen/thawed spermatozoa (60% motility) in the cranial part of each uterine horn. Seven days later, ovariohysterectomy was performed and the oviducts and uterine horns and body were flushed to recover embryos and unfertilized oocytes. In 80% of the bitches inseminated with fresh and 50% of bitches inseminated with frozen/thawed semen, embryos at 2 to 8 cells stage were recovered mostly from the, oviducts. This study indicates that pregnancies can be obtained with a single laparoscopic intrauterine insemination after single serum progesterone measurement, although with a low number of embryos. This result should be taken into account in case economic or logistic restrictions that affect the possibility of owners to plan an accurate monitoring of the optimal breeding time using fresh and frozen semen.en
dc.description.affiliationDepartamento de Cirurgia Veterinária Faculdade de Medicina Veterinária Universidade Federal de Uberlândia, MG
dc.description.affiliationPrograma de Pós-graduação Strictu Sensu em Biociência Animal Universidade de Cuiabá, MT
dc.description.affiliationDepartamento de Reprodução Animal Faculdade de Medicina Veterinária e Zootecnia Universidade Estadual Paulista, SP
dc.description.affiliationDepartamento de Cirurgia Veterinária Faculdade de Medicina Veterinária Centro Universitário de Rio Preto, SP
dc.description.affiliationDepartamento de Reprodução Animal Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista, SP
dc.description.affiliationDipartimento di Medicina Veterinaria e Scienze Animali Università Degli Studi di Milano, Lombardia
dc.description.affiliationUnespDepartamento de Reprodução Animal Faculdade de Medicina Veterinária e Zootecnia Universidade Estadual Paulista, SP
dc.description.affiliationUnespDepartamento de Reprodução Animal Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista, SP
dc.identifierhttp://dx.doi.org/10.1590/1984-3143-AR2022-0079
dc.identifier.citationAnimal Reproduction, v. 20, n. 1, 2023.
dc.identifier.doi10.1590/1984-3143-AR2022-0079
dc.identifier.issn1984-3143
dc.identifier.issn1806-9614
dc.identifier.scopus2-s2.0-85150282713
dc.identifier.urihttp://hdl.handle.net/11449/248525
dc.language.isoeng
dc.relation.ispartofAnimal Reproduction
dc.sourceScopus
dc.subjectbitches
dc.subjectembryo development
dc.subjectIntrauterine artificial insemination
dc.titleIn vivo embryo development in bitches inseminated laparoscopically after ovulation time estimated based on a single progesterone determinationen
dc.typeArtigo
unesp.author.orcid0000-0001-6591-7483[1]
unesp.author.orcid0000-0002-2202-0331[2]
unesp.author.orcid0000-0001-8360-0471[3]
unesp.author.orcid0000-0002-3321-6735[4]
unesp.author.orcid0000-0001-6118-7962[5]
unesp.author.orcid0000-0001-7696-0962[6]
unesp.author.orcid0000-0002-9598-9098[7]
unesp.departmentMedicina Veterinária Preventiva e Reprodução Animal - FCAVpt

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Artigos - Reprodução Animal e Radiologia Veterinária - FMVZ
Jaboticabal - FCAV - Faculdade de Ciências Agrárias e Veterinárias