Graphenic Aerogels Decorated with Ag Nanoparticles as 3D SERS Substrates for Biosensing

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dc.contributor.authorBiasotto, Glenda [UNESP]
dc.contributor.authorChiadò, Alessandro
dc.contributor.authorNovara, Chiara
dc.contributor.authorFontana, Marco
dc.contributor.authorArmandi, Marco
dc.contributor.authorZaghete, Maria Aparecida [UNESP]
dc.contributor.authorGiorgis, Fabrizio
dc.contributor.authorRivolo, Paola
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionPolitecnico di Torino
dc.contributor.institutionIIT-Istituto Italiano di Tecnologia
dc.date.accessioned2020-12-12T01:25:52Z
dc.date.available2020-12-12T01:25:52Z
dc.date.issued2020-07-01
dc.description.abstractA versatile and efficient surface-enhanced Raman scattering (SERS) substrate based on a hybrid aerogel composed of reduced graphene oxide (rGO) decorated with silver nanoparticles (AgNPs), suitable for highly sensitive label-free detection of chemical and biological species, is presented. The simple and low-cost one-pot hydrothermal synthesis allows obtaining of a 3D nanostructured spongy-like matrix that shows good spatial distribution of Ag nanoparticles in intimate contact with rGO flakes, characterized by means of several morphological, structural, and compositional techniques. The nanostructured material, tested by SERS analysis with both rhodamine 6G (R6G) and 4-mercaptobenzoic acid (MBA), shows a satisfying SERS efficiency, quantified in terms of minimum detectable concentration of 10−10 and 10−7 m, corresponding to on- and off-resonant excitation, respectively. The versatility of chemical/biochemical functionalization is successfully demonstrated by exploiting different routes, by immobilizing both protoporphyrin IX (PRPIX) and hemin (H) that take advantage of π−π non-covalent bonding with the graphene layers, as well as thiol-ended oligonucleotides (DNA probes/aptamers) directly grafted on the AgNPs. Finally, after the successful integration of the hybrid aerogel into a microfluidic chip, the biorecognition of miR222 is obtained demonstrating the reliability of the aerogel substrate as SERS platform for biosensing.en
dc.description.affiliationCDMF – Functional Materials Development Center Institute of Chemistry São Paulo State University-UNESP
dc.description.affiliationDISAT-Department of Applied Science and Technology Politecnico di Torino, C.so Duca degli Abruzzi 24
dc.description.affiliationCenter for Sustainable Future Technologies IIT-Istituto Italiano di Tecnologia, Via Livorno 60
dc.description.affiliationUnespCDMF – Functional Materials Development Center Institute of Chemistry São Paulo State University-UNESP
dc.description.sponsorshipRegione Piemonte
dc.identifierhttp://dx.doi.org/10.1002/ppsc.202000095
dc.identifier.citationParticle and Particle Systems Characterization, v. 37, n. 7, 2020.
dc.identifier.doi10.1002/ppsc.202000095
dc.identifier.issn1521-4117
dc.identifier.issn0934-0866
dc.identifier.scopus2-s2.0-85085934380
dc.identifier.urihttp://hdl.handle.net/11449/198932
dc.language.isoeng
dc.relation.ispartofParticle and Particle Systems Characterization
dc.sourceScopus
dc.subjectaerogels
dc.subjectbiosensing
dc.subjectgraphene
dc.subjectSERS
dc.subjectsilver nanoparticles
dc.titleGraphenic Aerogels Decorated with Ag Nanoparticles as 3D SERS Substrates for Biosensingen
dc.typeArtigo
unesp.author.orcid0000-0003-0672-5793[8]

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