Spatial Distribution of Heterochromatin Bodies in the Nuclei of Triatoma infestans (Klug)

dc.contributor.authorImperador, Carlos Henrique L.
dc.contributor.authorBardella, Vanessa B. [UNESP]
dc.contributor.authorDos Anjos, Eli Heber M.
dc.contributor.authorRodrigues, Vera L.C.C.
dc.contributor.authorCabral-De-Mello, Diogo C. [UNESP]
dc.contributor.authorMello, Maria Luiza S.
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionSuperintendence for Control of Endemic Diseases (SUCEN)
dc.date.accessioned2020-12-12T01:23:57Z
dc.date.available2020-12-12T01:23:57Z
dc.date.issued2020-06-01
dc.description.abstractConstitutive heterochromatin typically exhibits low gene density and is commonly found adjacent or close to the nuclear periphery, in contrast to transcriptionally active genes concentrated in the innermost nuclear region. In Triatoma infestans cells, conspicuous constitutive heterochromatin forms deeply stained structures named chromocenters. However, to the best of our knowledge, no information exists regarding whether these chromocenters acquire a precise topology in the cell nuclei or whether their 18S rDNA, which is important for ribosome function, faces the nuclear center preferentially. In this work, the spatial distribution of fluorescent Feulgen-stained chromocenters and the distribution of their 18S rDNA was analyzed in Malpighian tubule cells of T. infestans using confocal microscopy. The chromocenters were shown to be spatially positioned relatively close to the nuclear periphery, though not adjacent to it. The variable distance between the chromocenters and the nuclear periphery suggests mobility of these bodies within the cell nuclei. The distribution of 18S rDNA at the edge of the chromocenters was not found to face the nuclear interior exclusively. Because the genome regions containing 18S rDNA in the chromocenters also face the nuclear periphery, the proximity of the chromocenters to this nuclear region is not assumed to be associated with overall gene silencing.en
dc.description.affiliationDepartment of Structural and Functional Biology Institute of Biology University of Campinas (Unicamp), Rua Monteiro Lobato 255
dc.description.affiliationDepartment of Biology Institute of Biosciences State University of S o Paulo (Unesp), Avenida 24-A, 1515
dc.description.affiliationSuperintendence for Control of Endemic Diseases (SUCEN), Rua Afonso Pessini, 86
dc.description.affiliationUnespDepartment of Biology Institute of Biosciences State University of S o Paulo (Unesp), Avenida 24-A, 1515
dc.format.extent567-574
dc.identifierhttp://dx.doi.org/10.1017/S143192762000149X
dc.identifier.citationMicroscopy and Microanalysis, v. 26, n. 3, p. 567-574, 2020.
dc.identifier.doi10.1017/S143192762000149X
dc.identifier.issn1435-8115
dc.identifier.issn1431-9276
dc.identifier.scopus2-s2.0-85085074940
dc.identifier.urihttp://hdl.handle.net/11449/198863
dc.language.isoeng
dc.relation.ispartofMicroscopy and Microanalysis
dc.sourceScopus
dc.subject18S rDNA
dc.subjectchromatin topology
dc.subjectchromocenter
dc.subjectconfocal microscopy
dc.subjectFISH
dc.subjectheterochromatin
dc.titleSpatial Distribution of Heterochromatin Bodies in the Nuclei of Triatoma infestans (Klug)en
dc.typeArtigo

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