VARIABLE INTER-ASSAY ESTIMATION OF SPERM DNA FRAGMENTATION IN STALLIONS CLASSIFIED AS GOOD AND BAD SEMEN FREEZERS
| dc.contributor.author | Ferreira, H. N. | |
| dc.contributor.author | Ferreira-Silva, J. C. | |
| dc.contributor.author | Rocha, J. M. | |
| dc.contributor.author | Farras, M. C. [UNESP] | |
| dc.contributor.author | Calixto, M. | |
| dc.contributor.author | Moura, M. T. | |
| dc.contributor.author | Alvarenga, M. A. [UNESP] | |
| dc.contributor.author | Oliveira, M. A. L. | |
| dc.contributor.institution | Dept Med Vet UFRPE | |
| dc.contributor.institution | Fac Pio Decimo | |
| dc.contributor.institution | Universidade Estadual Paulista (Unesp) | |
| dc.date.accessioned | 2018-11-26T17:48:56Z | |
| dc.date.available | 2018-11-26T17:48:56Z | |
| dc.date.issued | 2018-01-01 | |
| dc.description.abstract | BACKGROUND: Semen cryopreservation causes DNA damage, thus requiring continuous monitoring. OBJECTIVE: To compare two assays for sperm DNA fragmentation (SDF) from stallions with contrasting semen freezability. MATERIALS AND METHODS: Thirteen stallions were classified as good semen freezers (GSF) or bad semen freezers (BSF). Ejaculates were cryopreserved with three diluents. Semen was subject to SDF evaluation using the sperm chromatin structure assay (SCSA) and Halomax (R) after thawing (0 h) and after a 4 h thermoresistance test. RESULTS: On semen of BSF, analysis by SCSA was similar between evaluations, but Halomax (R) showed increased SDF at 4 h. The GSF group was similar between time points in both assays. Diluents did not affect SDF, irrespective of the assay. Halomax (R) showed differences for BSF between time points, differently from SCSA. Linear regression did not show any correlation between assays. CONCLUSION: The use of Halomax (R) should be encouraged for sperm DNA fragmentation analysis in horse frozen-thawed semen, particularly under field conditions. | en |
| dc.description.affiliation | Dept Med Vet UFRPE, Lab Biotecnol Reprod, Recife, PE, Brazil | |
| dc.description.affiliation | Fac Pio Decimo, Aracaju, SE, Brazil | |
| dc.description.affiliation | Fac Med Vet & Zootecnia UNESP, Dept Reprod Anim & Radiol Vet, Lab Reprod Anim, Botucatu, SP, Brazil | |
| dc.description.affiliationUnesp | Fac Med Vet & Zootecnia UNESP, Dept Reprod Anim & Radiol Vet, Lab Reprod Anim, Botucatu, SP, Brazil | |
| dc.description.sponsorship | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) | |
| dc.description.sponsorship | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | |
| dc.format.extent | 67-71 | |
| dc.identifier.citation | Cryoletters. London: Cryo Letters, v. 39, n. 1, p. 67-71, 2018. | |
| dc.identifier.issn | 0143-2044 | |
| dc.identifier.uri | http://hdl.handle.net/11449/164055 | |
| dc.identifier.wos | WOS:000429076400008 | |
| dc.language.iso | eng | |
| dc.publisher | Cryo Letters | |
| dc.relation.ispartof | Cryoletters | |
| dc.relation.ispartofsjr | 0,234 | |
| dc.rights.accessRights | Acesso restrito | |
| dc.source | Web of Science | |
| dc.subject | reproduction | |
| dc.subject | cryobiology | |
| dc.subject | equine | |
| dc.subject | spermatozoa | |
| dc.title | VARIABLE INTER-ASSAY ESTIMATION OF SPERM DNA FRAGMENTATION IN STALLIONS CLASSIFIED AS GOOD AND BAD SEMEN FREEZERS | en |
| dc.type | Artigo | |
| dcterms.rightsHolder | Cryo Letters | |
| unesp.campus | Universidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária e Zootecnia, Botucatu | pt |
| unesp.department | Reprodução Animal e Radiologia Veterinária - FMVZ | pt |