Antimicrobial, antibiofilm and cytotoxic effects of a colloidal nanocarrier composed by chitosan-coated iron oxide nanoparticles loaded with chlorhexidine

dc.contributor.authorAraujo, Heitor Ceolin [UNESP]
dc.contributor.authorda Silva, Ana Carolina Gomes
dc.contributor.authorPaião, Luana Isabel
dc.contributor.authorMagario, Mychelle Keiko Watanabe
dc.contributor.authorFrasnelli, Sabrina Cruz Tfaile [UNESP]
dc.contributor.authorOliveira, Sandra Helena Penha de [UNESP]
dc.contributor.authorPessan, Juliano Pelim [UNESP]
dc.contributor.authorMonteiro, Douglas Roberto
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversity of Western São Paulo (UNOESTE)
dc.date.accessioned2020-12-12T02:20:13Z
dc.date.available2020-12-12T02:20:13Z
dc.date.issued2020-10-01
dc.description.abstractObjectives: This study evaluated the antimicrobial and antibiofilm effects of a colloidal nanocarrier for chlorhexidine (CHX) on Candida glabrata and Enterococcus faecalis, as well as tested its cytotoxic effect on murine fibroblasts. Methods: Iron oxide nanoparticles (IONPs) were coated with chitosan (CS) and loaded with CHX at 31.2, 78 and 156 μg/mL. Antimicrobial effects were assessed by determining the minimum inhibitory concentration (MIC), using the broth microdilution method, and fractional inhibitory concentration index (FICI). Preformed biofilms (48 h) were treated with different concentrations of the nanocarrier (24 h) and quantified by colony-forming units (CFUs), total biomass and metabolic activity. For cytotoxicity, the viability of L929 cells was evaluated by MTT assay after 24 and 48 h of exposure to the nanocarrier. Data were submitted to ANOVA and Fisher LSD or Tukey post-hoc tests (α = 0.05). Results: MIC and FICI results showed an indifferent interaction among the components of the nanocarrier for all strains evaluated. CHX alone and nanocarrier containing 156 μg/mL CHX did not differ from each other in reducing the number of CFUs. However, the nanocarrier containing 156 μg/mL CHX promoted the highest reductions in total biofilm biomass and metabolism, surpassing the effect of CHX alone. After 24 and 48 h of exposure, the nanocarrier reduced CHX toxicity to the L929 cell at low concentrations. Conclusion: These findings suggest that the CHX nanocarrier has potential to be used in the control of oral diseases associated with C. glabrata and E. faecalis. Clinical relevance: CHX has improved the antibiofilm effect and reduced the cytotoxicity (at low concentrations) when conjugated to CS-coated IONPs. This new colloidal formulation has potential as an alternative antimicrobial agent to pure CHX for the control of biofilm-related oral diseases, such as oral candidiasis and endodontic infections.en
dc.description.affiliationSão Paulo State University (Unesp) School of Dentistry Araçatuba Department of Preventive and Restorative Dentistry, Araçatuba
dc.description.affiliationSchool of Dentistry Presidente Prudente University of Western São Paulo (UNOESTE), Presidente Prudente
dc.description.affiliationSão Paulo State University (Unesp) School of Dentistry Araçatuba Department of Basic Sciences, Araçatuba
dc.description.affiliationGraduate Program in Dentistry (GPD - Master's Degree) University of Western São Paulo (UNOESTE), Presidente Prudente
dc.description.affiliationUnespSão Paulo State University (Unesp) School of Dentistry Araçatuba Department of Preventive and Restorative Dentistry, Araçatuba
dc.description.affiliationUnespSão Paulo State University (Unesp) School of Dentistry Araçatuba Department of Basic Sciences, Araçatuba
dc.identifierhttp://dx.doi.org/10.1016/j.jdent.2020.103453
dc.identifier.citationJournal of Dentistry, v. 101.
dc.identifier.doi10.1016/j.jdent.2020.103453
dc.identifier.issn0300-5712
dc.identifier.scopus2-s2.0-85089804922
dc.identifier.urihttp://hdl.handle.net/11449/200945
dc.language.isoeng
dc.relation.ispartofJournal of Dentistry
dc.sourceScopus
dc.subjectBiofilms
dc.subjectCandida
dc.subjectChlorhexidine
dc.subjectCytotoxicity
dc.subjectEnterococcus faecalis
dc.subjectIron oxide nanoparticles
dc.titleAntimicrobial, antibiofilm and cytotoxic effects of a colloidal nanocarrier composed by chitosan-coated iron oxide nanoparticles loaded with chlorhexidineen
dc.typeArtigo
unesp.author.orcid0000-0001-5749-592X[1]
unesp.author.orcid0000-0002-1550-3933[7]
unesp.author.orcid0000-0001-5229-5259[8]

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