Flies (Insecta, Diptera) collected in the environment of dairy farms as carriers of Rotavirus A and betacoronavirus

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dc.contributor.authorBertolini, Amanda Bezerra [UNESP]
dc.contributor.authorThyssen, Patrícia Jacqueline
dc.contributor.authorBrandão, Paulo Eduardo
dc.contributor.authorPrado, Aline Marrara
dc.contributor.authorSilva, Sheila Oliveira de Souza
dc.contributor.authorMioni, Mateus de Souza Ribeiro [UNESP]
dc.contributor.authorde Gouvea, Fábio Lucas Rezende [UNESP]
dc.contributor.authorPantoja, José Carlos de Figueiredo [UNESP]
dc.contributor.authorLangoni, Helio [UNESP]
dc.contributor.authorGuimarães, Felipe Freitas [UNESP]
dc.contributor.authorJoaquim, Sâmea Fernandes [UNESP]
dc.contributor.authorGuerra, Simony Trevizan [UNESP]
dc.contributor.authorLeite, Domingos da Silva
dc.contributor.authorRall, Vera Mores [UNESP]
dc.contributor.authorHernandes, Rodrigo Tavanelli [UNESP]
dc.contributor.authorLucheis, Simone Baldini [UNESP]
dc.contributor.authorRossi, Gabriel Augusto Marques
dc.contributor.authorRibeiro, Márcio Garcia [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2023-07-29T13:44:08Z
dc.date.available2023-07-29T13:44:08Z
dc.date.issued2023-03-01
dc.description.abstractAIMS: We aimed to investigate the prevalence of rotavirus and coronavirus in dipterans that commonly inhabit the environment of dairy farms. METHODS AND RESULTS: We collected 217 insect specimens from nine dairy farms, which were examined through hemi-nested RT-PCR followed by Sanger sequencing in search of VP1 and N genes for rotavirus and bovine coronavirus-BCoV, respectively. With a predominance of Muscidae (152/217 = 70%) 11 families of Diptera were identified. Rotavirus A (RVA) and betacoronavirus (BCoV) were detected in 14.7% (32/217) and 4.6% (10/217) of the dipterans, respectively. Sequencing of the amplicons was possible for 11.5% (25/217) of RVA and 0.5% (1/217) of BCoV, confirming the presence of these pathogens. CONCLUSIONS: Our findings highlight the role of dipterans as carriers of RVA and BCoV of great relevance for public and animal health.en
dc.description.affiliationDepartment of Animal Production and Preventive Veterinary Medicine School of Veterinary Medicine and Animal Sciences São Paulo State University-UNESP
dc.description.affiliationDepartment of Animal Biology Institute of Biology University of Campinas-UNICAMP
dc.description.affiliationDepartment of Preventive Veterinary Medicine and Animal Health School of Veterinary Medicine and Animal Science University of São Paulo FMVZ-USP
dc.description.affiliationDepartment of Genetics Evolution Microbiology Immunology, UNICAMP
dc.description.affiliationDepartment of Chemical and Biological Sciences Institute of Biosciences UNESP
dc.description.affiliationVila Velha University (UVV), Faculty of Veterinary Medicine, Department of Veterinary Medicine, 29102-920 Vila Velha, ES, Brazil
dc.description.affiliationUnespDepartment of Animal Production and Preventive Veterinary Medicine School of Veterinary Medicine and Animal Sciences São Paulo State University-UNESP
dc.description.affiliationUnespDepartment of Chemical and Biological Sciences Institute of Biosciences UNESP
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2015/19688-8
dc.identifierhttp://dx.doi.org/10.1093/jambio/lxad020
dc.identifier.citationJournal of applied microbiology, v. 134, n. 3, 2023.
dc.identifier.doi10.1093/jambio/lxad020
dc.identifier.issn1365-2672
dc.identifier.scopus2-s2.0-85149182515
dc.identifier.urihttp://hdl.handle.net/11449/248443
dc.language.isoeng
dc.relation.ispartofJournal of applied microbiology
dc.sourceScopus
dc.subjectBCoV
dc.subjectdairy cows
dc.subjectflying insects
dc.subjectRVA
dc.subjectVector-borne diseases
dc.titleFlies (Insecta, Diptera) collected in the environment of dairy farms as carriers of Rotavirus A and betacoronavirusen
dc.typeArtigo
unesp.author.orcid0000-0002-1575-6770[1]
unesp.author.orcid0000-0001-7343-2419[2]
unesp.author.orcid0000-0001-7967-7628[17]

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Artigos - Produção Animal - FMVZ