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Differential Expression of Monocarboxylate Transporter 1 and Ancillary Protein CD147 in Red Blood Cells of Show Jumping Horses

dc.contributor.authorJúnior, Walter Heinz Feringer [UNESP]
dc.contributor.authorGarcia de Carvalho, Julia Ribeiro [UNESP]
dc.contributor.authorMendes de Almeida, Maria Luiza [UNESP]
dc.contributor.authorMacedo Lemos, Eliana Gertrudes [UNESP]
dc.contributor.authorBrioschi Soares, Otávio Augusto
dc.contributor.authorRibeiro, Gesiane
dc.contributor.authorde Queiroz-Neto, Antonio [UNESP]
dc.contributor.authorde Camargo Ferraz, Guilherme [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionBrazilain Army
dc.contributor.institutionHOVET
dc.date.accessioned2020-12-12T01:40:30Z
dc.date.available2020-12-12T01:40:30Z
dc.date.issued2019-10-01
dc.description.abstractWe compare the expression levels of the lactate transporter complex consisting of the lactate transporter, monocarboxylate transporter 1 (MCT1), and its ancillary protein, cluster of differentiation 147 (CD147), in the membranes of red blood cells (RBCs) from two breeds of jumping horses and associate the expression levels of these proteins with their jumping ability. The expression levels of MCT1 and CD147 proteins on the membranes of RBCs collected from 30 show jumping horses of two different breeds were quantified: the Brazilian Sport Horses (n = 17) and the European Warmbloods (n = 13). The levels of MCT1 and CD147 in the RBC membranes were measured by Western blot using horse-specific antibodies. Statistical analyses included unpaired Student t-test and Chi-squared test. According to the expression levels of MCT1 and CD147 proteins, 88% of the Brazilian Sport Horses were categorized as high lactate transporters and the remaining 12% as low lactate transporters. The opposite was found for the European Warmbloods, where most animals (77%) were classified as low lactate transporters and the remaining animals (23%) were classified as high lactate transporters. Brazilian Sport Horses express statistically significantly higher levels of CD147 and MCT1 than European Warmbloods. The classification of horses considering the expression of proteins involved in the ability to transport lactate through the complex MCT1-CD147 seems to be breed dependent, with horses that are able to jump higher obstacles showing lower expression of the MCT1-CD147 complex in their RBCs.en
dc.description.affiliationDepartamento de Morfologia e Fisiologia Animal Faculdades de Ciências Agrárias e Veterinárias UNESP Univ Estadual Paulista Campus de Jaboticabal Laboratório de Farmacologia e Fisiologia do Exercício Equino (LAFEQ) Jaboticabal
dc.description.affiliationDepartamento de Tecnologia Faculdades de Ciências Agrárias e Veterinárias UNESP Univ Estadual Paulista Campus de Jaboticabal
dc.description.affiliationBrazilain Army
dc.description.affiliationFaculdades Metropolitanas Unidas HOVET
dc.description.affiliationUnespDepartamento de Morfologia e Fisiologia Animal Faculdades de Ciências Agrárias e Veterinárias UNESP Univ Estadual Paulista Campus de Jaboticabal Laboratório de Farmacologia e Fisiologia do Exercício Equino (LAFEQ) Jaboticabal
dc.description.affiliationUnespDepartamento de Tecnologia Faculdades de Ciências Agrárias e Veterinárias UNESP Univ Estadual Paulista Campus de Jaboticabal
dc.identifierhttp://dx.doi.org/10.1016/j.jevs.2019.102791
dc.identifier.citationJournal of Equine Veterinary Science, v. 81.
dc.identifier.doi10.1016/j.jevs.2019.102791
dc.identifier.issn0737-0806
dc.identifier.scopus2-s2.0-85072884580
dc.identifier.urihttp://hdl.handle.net/11449/199462
dc.language.isoeng
dc.relation.ispartofJournal of Equine Veterinary Science
dc.sourceScopus
dc.subjectCD 147
dc.subjectExercise physiology
dc.subjectLactate
dc.subjectLactate transporter complex
dc.subjectMCT1
dc.titleDifferential Expression of Monocarboxylate Transporter 1 and Ancillary Protein CD147 in Red Blood Cells of Show Jumping Horsesen
dc.typeArtigo
unesp.departmentMorfologia e Fisiologia Animal - FCAVpt
unesp.departmentTecnologia - FCAVpt

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