Antibacterial action and target mechanisms of zinc oxide nanoparticles against bacterial pathogens

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dc.contributor.authorMendes, Carolina Rosai [UNESP]
dc.contributor.authorDilarri, Guilherme [UNESP]
dc.contributor.authorForsan, Carolina Froes [UNESP]
dc.contributor.authorSapata, Vinícius de Moraes Ruy [UNESP]
dc.contributor.authorLopes, Paulo Renato Matos [UNESP]
dc.contributor.authorde Moraes, Peterson Bueno
dc.contributor.authorMontagnolli, Renato Nallin [UNESP]
dc.contributor.authorFerreira, Henrique [UNESP]
dc.contributor.authorBidoia, Ederio Dino [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)
dc.date.accessioned2022-05-01T13:41:39Z
dc.date.available2022-05-01T13:41:39Z
dc.date.issued2022-12-01
dc.description.abstractZinc oxide nanoparticles (ZnO NPs) are one of the most widely used nanoparticulate materials due to their antimicrobial properties, but their main mechanism of action (MOA) has not been fully elucidated. This study characterized ZnO NPs by using X-ray diffraction, FT-IR spectroscopy and scanning electron microscopy. Antimicrobial activity of ZnO NPs against the clinically relevant bacteria Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and the Gram-positive model Bacillus subtilis was evaluated by performing resazurin microtiter assay (REMA) after exposure to the ZnO NPs at concentrations ranging from 0.2 to 1.4 mM. Sensitivity was observed at 0.6 mM for the Gram-negative and 1.0 mM for the Gram-positive cells. Fluorescence microscopy was used to examine the interference of ZnO NPs on the membrane and the cell division apparatus of B. subtilis (amyen
dc.description.affiliationDepartment of General and Applied Biology Sao Paulo State University (UNESP), 24-A Avenue, 1515, SP
dc.description.affiliationCollege of Technology and Agricultural Sciences Sao Paulo State University (UNESP), SP‑294, km 651, SP
dc.description.affiliationSchool of Technology State University of Campinas (UNICAMP), 6122, SP
dc.description.affiliationDepartment of Natural Sciences Mathematics and Education Agricultural Sciences Centre Federal University of Sao Carlos (UFSCar), SP-330, km 174, SP
dc.description.affiliationUnespDepartment of General and Applied Biology Sao Paulo State University (UNESP), 24-A Avenue, 1515, SP
dc.description.affiliationUnespCollege of Technology and Agricultural Sciences Sao Paulo State University (UNESP), SP‑294, km 651, SP
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2015/50162-2
dc.description.sponsorshipIdFAPESP: 2017/07306-9
dc.identifierhttp://dx.doi.org/10.1038/s41598-022-06657-y
dc.identifier.citationScientific Reports, v. 12, n. 1, 2022.
dc.identifier.doi10.1038/s41598-022-06657-y
dc.identifier.issn2045-2322
dc.identifier.scopus2-s2.0-85124777921
dc.identifier.urihttp://hdl.handle.net/11449/234152
dc.language.isoeng
dc.relation.ispartofScientific Reports
dc.sourceScopus
dc.titleAntibacterial action and target mechanisms of zinc oxide nanoparticles against bacterial pathogensen
dc.typeArtigo

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Artigos - Ciências Biológicas - FCFAR
Artigos - Bioquímica e Microbiologia - IBRC