Neonatal immune response of Brazilian beef cattle to vaccination with Clostridium botulinum toxoids types C and D by indirect ELISA

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dc.contributor.authorCurci, V. C. M.
dc.contributor.authorNogueira, A. H. C.
dc.contributor.authorNobrega, F. L. C. [UNESP]
dc.contributor.authorAraujo, R. F. [UNESP]
dc.contributor.authorPerri, S. H. [UNESP]
dc.contributor.authorCardoso, T. C. [UNESP]
dc.contributor.authorDutra, Iveraldo dos Santos [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionAgência Paulista de Tecnologia dos Agronegócios (APTA)
dc.date.accessioned2014-05-20T15:32:16Z
dc.date.available2014-05-20T15:32:16Z
dc.date.issued2010-01-01
dc.description.abstractTypes C and D strains of Clostridium botulinum are commonly related to avian and mammalian botulism. Although there are numerous vaccine recommendations, little research has been conducted to indicate the real effectiveness of vaccine timing or the ideal immunization protocol for young beef calves. Four commercially available vaccines, two bivalent (Clostridium botulinum types C and D; vaccines 1 and 2) and two polyvalent (all Clostridium spp. including Clostridium botulinum types C and D; vaccines 3 and 4), that are currently used in Brazilian herds, were tested in order to verify the maternal immune response. One hundred cows, divided into four vaccinated groups and one unvaccinated group, were given a two-dose subcutaneous immunization, at day zero, followed by a second dose given at 42 days post-vaccination, which corresponded to 40 days before birth. Serum samples (n = 75) were collected only from healthy neonatal calves at 0, 7, 45 and 90 days post-calving (DPC) and subjected to indirect ELISA using the purified C and D holotoxins as capture antigens. The serological profile showed that all vaccines were able to induce a satisfactory neonatal immune response to both holotoxins at 7 DPC. However, at 45 and 90 DPC, a significant reduction (p < 0.05) was observed in the antibody level against C and D holotoxins in all tested vaccines. Neonatal immunization in calves is compromised by significant levels of maternal antibodies so that the necessity of planning a calf vaccination program involves assessment of disease risks at the production site. Finally, our findings represent the first demonstration of maternal immunity transferred to neonatal beef calves, including immunity levels after vaccination against Clostridium botulinum toxoids C and D.en
dc.description.affiliationUNESP Univ Estadual Paulista, São Paulo State Univ, Sch Vet Med, Lab Clostridiosis, Aracatuba, SP, Brazil
dc.description.affiliationUNESP Univ Estadual Paulista, São Paulo State Univ, Sch Vet Med, Virol Lab, Aracatuba, SP, Brazil
dc.description.affiliationAPTA, São Paulo Agcy Agribusiness Technol, Res & Dev Unit, Aracatuba, SP, Brazil
dc.description.affiliationUnespUNESP Univ Estadual Paulista, São Paulo State Univ, Sch Vet Med, Lab Clostridiosis, Aracatuba, SP, Brazil
dc.description.affiliationUnespUNESP Univ Estadual Paulista, São Paulo State Univ, Sch Vet Med, Virol Lab, Aracatuba, SP, Brazil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent509-513
dc.identifierhttp://dx.doi.org/10.1590/S1678-91992010000300018
dc.identifier.citationJournal of Venomous Animals and Toxins Including Tropical Diseases. Botucatu: Cevap-unesp, v. 16, n. 3, p. 509-513, 2010.
dc.identifier.fileS1678-91992010000300018-en.pdf
dc.identifier.issn1678-9199
dc.identifier.lattes6247817803779719
dc.identifier.scieloS1678-91992010000300018
dc.identifier.urihttp://hdl.handle.net/11449/41214
dc.identifier.wosWOS:000281692600018
dc.language.isoeng
dc.publisherUniversidade Estadual Paulista (Unesp), Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP)
dc.relation.ispartofJournal of Venomous Animals and Toxins Including Tropical Diseases
dc.relation.ispartofjcr1.782
dc.relation.ispartofsjr0,573
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectClostridium botulinumen
dc.subjectneonatal immunityen
dc.subjectcattleen
dc.subjectbotulismen
dc.subjectBrazilen
dc.titleNeonatal immune response of Brazilian beef cattle to vaccination with Clostridium botulinum toxoids types C and D by indirect ELISAen
dc.typeTrabalho apresentado em evento
dcterms.licensehttp://www.scielo.br/revistas/jvatitd/iaboutj.htm
dcterms.rightsHolderCevap-unesp
unesp.author.lattes6247817803779719
unesp.author.orcid0000-0001-5083-5557[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária, Araçatubapt

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