Lack of Population Genetic Structuring in Ocelots (Leopardus pardalis) in a Fragmented Landscape

dc.contributor.authorFigueiredo, Marina G. [UNESP]
dc.contributor.authorCervini, Marcelo
dc.contributor.authorRodrigues, Fernando P.
dc.contributor.authorEizirik, Eduardo
dc.contributor.authorAzevedo, Fernando C.C.
dc.contributor.authorCullen, Laury
dc.contributor.authorCrawshaw, Peter G.
dc.contributor.authorGaletti, Pedro M.
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade Estadual do Sudoeste da Bahia
dc.contributor.institutionUniversidade de Brasília (UnB)
dc.contributor.institutionLaboratório de Biologia Genômica e Molecular
dc.contributor.institutionInstituto Pró-Carnívoros
dc.contributor.institutionUniversidade Federal de São João del-Rei
dc.contributor.institutionInstituto de Pesquisas Ecológicas
dc.contributor.institutionInstituto Chico Mendes de Conservação da Biodiversidade
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)
dc.description.abstractHabitat fragmentation can promote patches of small and isolated populations, gene flow disruption between those populations, and reduction of local and total genetic variation. As a consequence, these small populations may go extinct in the long-term. The ocelot (Leopardus pardalis), originally distributed from Texas to southern Brazil and northern Argentina, has been impacted by habitat fragmentation throughout much of its range. To test whether habitat fragmentation has already induced genetic differentiation in an area where this process has been documented for a larger felid (jaguars), we analyzed molecular variation in ocelots inhabiting two Atlantic Forest fragments, Morro do Diabo (MD) and Iguaçu Region (IR). Analyses using nine microsatellites revealed mean observed and expected heterozygosity of 0.68 and 0.70, respectively. The MD sampled population showed evidence of a genetic bottleneck under two mutational models (TPM = 0.03711 and SMM = 0.04883). Estimates of genetic structure (FST = 0.027; best fit of k = 1 with STRUCTURE) revealed no meaningful differentiation between these populations. Thus, our results indicate that the ocelot populations sampled in these fragments are still not significantly different genetically, a pattern that strongly contrasts with that previously observed in jaguars for the same comparisons. This observation is likely due to a combination of two factors: (i) larger effective population size of ocelots (relative to jaguars) in each fragment, implying a slower effect of drift-induced differentiation; and (ii) potentially some remaining permeability of the anthropogenic matrix for ocelots, as opposed to the observed lack of permeability for jaguars. The persistence of ocelot gene flow between these areas must be prioritized in long-term conservation planning on behalf of these felids.en
dc.description.affiliationUniversidade Estadual Paulista Júlio de Mesquita Filho Departamento de Zootecnia, Via de Acesso Paulo Donato Castellane, s/n
dc.description.affiliationUniversidade Estadual do Sudoeste da Bahia Departamento de Ciências Biológicas, Av. José Moreira Sobrinho, s/n
dc.description.affiliationUniversidade de Brasília Instituto de Ciências Biológicas Departamento de Genética e Morfologia, Campus Universitário Darcy Ribeiro
dc.description.affiliationPUCRS Faculdade de Biociências Laboratório de Biologia Genômica e Molecular, Av. Ipiranga 6681, Prédio 12
dc.description.affiliationInstituto Pró-Carnívoros
dc.description.affiliationUniversidade Federal de São João Del-Rei Departamento de Ciências Naturais Universidade Federal de São João del-Rei, Campus Dom Bosco
dc.description.affiliationInstituto de Pesquisas Ecológicas, Rua Ricardo Fogarolli, 387, Vila Sao Paulo
dc.description.affiliationCentro Nacional de Pesquisa Para a Conservação de Predadores Naturais Instituto Chico Mendes de Conservação da Biodiversidade, Avenida dos Bandeirantes, s/n Balneário Municipal
dc.description.affiliationLaboratório de Biodiversidade Molecular e Citogenética Universidade Federal de São Carlos Departamento de Genética e Evolução, Via Washington Luis, km 235, Caixa Postal 676
dc.description.affiliationUnespUniversidade Estadual Paulista Júlio de Mesquita Filho Departamento de Zootecnia, Via de Acesso Paulo Donato Castellane, s/n
dc.identifier.citationDiversity, v. 7, n. 3, p. 295-306, 2015.
dc.subjectGenetic diversity
dc.subjectHabitat fragmentation
dc.titleLack of Population Genetic Structuring in Ocelots (Leopardus pardalis) in a Fragmented Landscapeen