The Effect of Photobiomodulation Therapy in Different Doses on Bone Repair of Critical Size Defects in Rats: A Histomorphometric Study

Página do item simplificado
dc.contributor.authorde Marco, Andrea Carvalho [UNESP]
dc.contributor.authorTorquato, Letícia Cavassini [UNESP]
dc.contributor.authorGonçalves, Paulo Roberto
dc.contributor.authorRibeiro, Tatiana Camacho
dc.contributor.authorNunes, Camilla Moretto [UNESP]
dc.contributor.authorBernardo, Daniella Vicensotto
dc.contributor.authorGomes, Mônica Fernandes [UNESP]
dc.contributor.authorJardini, Maria Aparecida Neves [UNESP]
dc.contributor.authorSantamaria, Mauro Pedrine [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionSao Jose dos Campos
dc.date.accessioned2022-05-01T11:07:21Z
dc.date.available2022-05-01T11:07:21Z
dc.date.issued2021-01-01
dc.description.abstractIntroduction: Photobiomodulation therapy (PBM) appears to induce osteogenesis and stimulate fracture repair; because of its capacity, it is considered a promising treatment, but the characteristics of response to different radiation doses must be investigated through in vivo studies to establish their safety and effectiveness. Thus, this paper aims to analyze the effects of the PBM at different doses on the repair of critical bone defects through histological and histomorphometric analyses. Methods: Sixty 90-day-old adult rats (Rattus norvegicus, albinus, Wistar) weighing approximately 300 g were used. Critical bone defects of 5 mm in diameter were performed in their calvaria. The animals were randomly separated into 5 groups: C-Blood clot, L15-PBM 15J/cm2, L30-PBM 30 J/cm2, L45-PBM 45 J/cm2, L60-PBM 60 J/cm2. Each group was subdivided according to observation periods of 30 and 60 days with 6 rats in each subgroup. Low-level gallium aluminum arsenide (GaAlAs) lasers were used at a 660 nm wavelength, 30 mW and 0.04 cm2 in area. The PBM was applied over 5 points; 4 points of application were distributed on the edges while one point of application was located in the center of the bone defect. PBM occurred right after the procedure. In 30 and 60 days, the animals were euthanized by anesthesia overdose and the analyses were performed. The data were analyzed statistically by the ANOVA, together with the Tukey test, whose significance level was 5%. Results: As regards the treatment factor, the highest percentage of bone neoformation was achieved by group L45-60. The group with the highest closure, despite not having a statistically significant difference with the other doses, was 45 J with only 0.49 mm between edges. Conclusion: Thus, the present study allowed concluding that the highest percentage of bone neoformation area was achieved at 45 J/cm2 in 60 days; that is, it was significantly effective in comparison with other doses.en
dc.description.affiliationDepartment of Diagnosis and Surgery – Division of Periodontology São Paulo State University (UNESP) Institute of Science and Tecnology
dc.description.affiliationSao Jose dos Campos
dc.description.affiliationDepartment of Biosciences and Oral Diagnosis São Paulo State University (UNESP) Institute of Science and Technology
dc.description.affiliationUnespDepartment of Diagnosis and Surgery – Division of Periodontology São Paulo State University (UNESP) Institute of Science and Tecnology
dc.description.affiliationUnespDepartment of Biosciences and Oral Diagnosis São Paulo State University (UNESP) Institute of Science and Technology
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2015/10376-3
dc.format.extent1-9
dc.identifierhttp://dx.doi.org/10.34172/jlms.2021.53
dc.identifier.citationJournal of Lasers in Medical Sciences, v. 12, n. 1, p. 1-9, 2021.
dc.identifier.doi10.34172/jlms.2021.53
dc.identifier.issn2228-6721
dc.identifier.issn2008-9783
dc.identifier.scopus2-s2.0-85120361632
dc.identifier.urihttp://hdl.handle.net/11449/233859
dc.language.isoeng
dc.relation.ispartofJournal of Lasers in Medical Sciences
dc.sourceScopus
dc.subjectBone Regeneration
dc.subjectLasers
dc.subjectLow-Level Light Therapy
dc.subjectRats
dc.titleThe Effect of Photobiomodulation Therapy in Different Doses on Bone Repair of Critical Size Defects in Rats: A Histomorphometric Studyen
dc.typeArtigo
unesp.author.orcid0000-0002-1894-7855[1]
unesp.author.orcid0000-0003-0962-156X[2]
unesp.author.orcid0000-0002-6189-3362[3]
unesp.author.orcid0000-0003-4844-2724[4]
unesp.author.orcid0000-0002-5399-0314[5]
unesp.author.orcid0000-0002-7999-8947[6]
unesp.author.orcid0000-0003-3358-5116[7]
unesp.author.orcid0000-0003-3229-8019[8]
unesp.author.orcid0000-0001-9468-0729[9]

Arquivos

Coleções

Artigos - Biociências e Diagnóstico Bucal - ICT
Artigos - Diagnóstico e Cirurgia - ICT