Genetic variability among the wild boars (Sus scrofa scrofa), crossbred animals and pigs using microsatellite markers (STRs)

Silva, Paula Viana Correa da [UNESP]; Lui, Jeffrey Frederico [UNESP]; Band, Guilherme de Oliveira [UNESP]; Regitano, Luciana Correia de Almeida; Grossi, Selma de Fátima; Sollero, Bruna Pena; Nunes, Cleujosí da Silva

Genetic variability among the wild boars (Sus scrofa scrofa), crossbred animals and pigs using microsatellite markers (STRs)

Arquivos

S1516-89132011000200011.pdf (56.19 KB)

Data

2011-04-01

Autores

Silva, Paula Viana Correa da

Lui, Jeffrey Frederico

Band, Guilherme de Oliveira

Regitano, Luciana Correia de Almeida

Grossi, Selma de Fátima

Sollero, Bruna Pena

Nunes, Cleujosí da Silva

Editor

Brazilian Archives of Biology and Technology

Tipo

Artigo

Direito de acesso

Acesso aberto

Resumo

The aim of this work was to study the genetic variability among the wild boars, crossbred animals and pigs using microsatellite markers. Five genetic groups were studied. The fragments of three microsatellites developed for Sus scrofa domestica - IGF1, ACTG2 and TNFB - were amplified through PCR technique to evaluate the expected intra populacion variability (He) and observed (Ho) heterozygosity, and endogamy coefficient (F IS ) within each population and inter population variability F IS , testing relationship among five genetic groups to establish the genetic distance among them. The high level of observed heterozygosity values varied between 0.537 and 0.7871. Generally, F IS was low, suggesting that the endogamy did not exist between the tested animals.