Cell-line-dependent crystal morphology and sublocalization of the Thyrinteina arnobia cypovirus polyhedrin expressed from a recombinant baculovirus
dc.contributor.author | Silva, Leonardo A. | |
dc.contributor.author | Ardisson-Araújo, Daniel M. P. | |
dc.contributor.author | Morgado, Fabricio S. | |
dc.contributor.author | Horta, André B. [UNESP] | |
dc.contributor.author | Lemos, Manoel Victor Franco [UNESP] | |
dc.contributor.author | Wilcken, Carlos F. [UNESP] | |
dc.contributor.author | Ribeiro, Bergmann M. | |
dc.contributor.institution | UnB-University of Brasília | |
dc.contributor.institution | Federal University of Santa Maria | |
dc.contributor.institution | Universidade Estadual Paulista (Unesp) | |
dc.date.accessioned | 2019-10-06T16:25:17Z | |
dc.date.available | 2019-10-06T16:25:17Z | |
dc.date.issued | 2019-06-01 | |
dc.description.abstract | We describe an unexpected feature observed for the heterologous expression of the Thyrinteina arnobia cypovirus polyhedrin from a recombinant baculovirus infection in different insect cell lines. The in cellulo-formed crystals varied in size and shape depending on the cell line. Crystals formed in Trichoplusia ni-derived cells were cubic (0.1-2 μm) and localized in both the nucleus and cytoplasm, whereas those formed in Spodoptera frugiperda-derived cells were ovate and ellipsoidal (0.1-3 μm) and also localized in both the nucleus and cytoplasm. The molecular basis for differences in the morphology, size, and location of cypovirus occlusion bodies is unclear, and cellular proteins might play a role in their formation and location. | en |
dc.description.affiliation | Cell Biology Department UnB-University of Brasília | |
dc.description.affiliation | Laboratory of Insect Virology Department of Biochemistry and Molecular Biology Federal University of Santa Maria | |
dc.description.affiliation | Plant Protection Department FCA/UNESP São Paulo State University, Campus of Botucatu | |
dc.description.affiliation | Applied Biology Department FCAV/UNESP São Paulo State University, Campus of Jaboticabal | |
dc.description.affiliationUnesp | Plant Protection Department FCA/UNESP São Paulo State University, Campus of Botucatu | |
dc.description.affiliationUnesp | Applied Biology Department FCAV/UNESP São Paulo State University, Campus of Jaboticabal | |
dc.description.sponsorship | Fundação de Apoio à Pesquisa do Distrito Federal | |
dc.description.sponsorship | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | |
dc.description.sponsorship | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) | |
dc.description.sponsorshipId | Fundação de Apoio à Pesquisa do Distrito Federal: 193.001532/2016 | |
dc.description.sponsorshipId | CNPq: 407908/2013-7 | |
dc.description.sponsorshipId | CNPq: 428799/2018-3 | |
dc.description.sponsorshipId | CAPES: CAPES/PROEX 23038.005848/2018-31 | |
dc.format.extent | 1677-1682 | |
dc.identifier | http://dx.doi.org/10.1007/s00705-019-04214-z | |
dc.identifier.citation | Archives of Virology, v. 164, n. 6, p. 1677-1682, 2019. | |
dc.identifier.doi | 10.1007/s00705-019-04214-z | |
dc.identifier.issn | 0304-8608 | |
dc.identifier.lattes | 7179273060624761 | |
dc.identifier.scopus | 2-s2.0-85064200276 | |
dc.identifier.uri | http://hdl.handle.net/11449/188961 | |
dc.language.iso | eng | |
dc.relation.ispartof | Archives of Virology | |
dc.rights.accessRights | Acesso aberto | |
dc.source | Scopus | |
dc.title | Cell-line-dependent crystal morphology and sublocalization of the Thyrinteina arnobia cypovirus polyhedrin expressed from a recombinant baculovirus | en |
dc.type | Artigo | |
unesp.author.lattes | 7179273060624761 | |
unesp.author.lattes | 7353607022049208[6] | |
unesp.author.orcid | 0000-0002-6292-7990[2] | |
unesp.author.orcid | 0000-0001-9875-4158[6] | |
unesp.department | Proteção Vegetal - FCA | pt |
unesp.department | Biologia - FCAV | pt |