Cell-line-dependent crystal morphology and sublocalization of the Thyrinteina arnobia cypovirus polyhedrin expressed from a recombinant baculovirus

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dc.contributor.authorSilva, Leonardo A.
dc.contributor.authorArdisson-Araújo, Daniel M. P.
dc.contributor.authorMorgado, Fabricio S.
dc.contributor.authorHorta, André B. [UNESP]
dc.contributor.authorLemos, Manoel Victor Franco [UNESP]
dc.contributor.authorWilcken, Carlos F. [UNESP]
dc.contributor.authorRibeiro, Bergmann M.
dc.contributor.institutionUnB-University of Brasília
dc.contributor.institutionFederal University of Santa Maria
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2019-10-06T16:25:17Z
dc.date.available2019-10-06T16:25:17Z
dc.date.issued2019-06-01
dc.description.abstractWe describe an unexpected feature observed for the heterologous expression of the Thyrinteina arnobia cypovirus polyhedrin from a recombinant baculovirus infection in different insect cell lines. The in cellulo-formed crystals varied in size and shape depending on the cell line. Crystals formed in Trichoplusia ni-derived cells were cubic (0.1-2 μm) and localized in both the nucleus and cytoplasm, whereas those formed in Spodoptera frugiperda-derived cells were ovate and ellipsoidal (0.1-3 μm) and also localized in both the nucleus and cytoplasm. The molecular basis for differences in the morphology, size, and location of cypovirus occlusion bodies is unclear, and cellular proteins might play a role in their formation and location.en
dc.description.affiliationCell Biology Department UnB-University of Brasília
dc.description.affiliationLaboratory of Insect Virology Department of Biochemistry and Molecular Biology Federal University of Santa Maria
dc.description.affiliationPlant Protection Department FCA/UNESP São Paulo State University, Campus of Botucatu
dc.description.affiliationApplied Biology Department FCAV/UNESP São Paulo State University, Campus of Jaboticabal
dc.description.affiliationUnespPlant Protection Department FCA/UNESP São Paulo State University, Campus of Botucatu
dc.description.affiliationUnespApplied Biology Department FCAV/UNESP São Paulo State University, Campus of Jaboticabal
dc.description.sponsorshipFundação de Apoio à Pesquisa do Distrito Federal
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIdFundação de Apoio à Pesquisa do Distrito Federal: 193.001532/2016
dc.description.sponsorshipIdCNPq: 407908/2013-7
dc.description.sponsorshipIdCNPq: 428799/2018-3
dc.description.sponsorshipIdCAPES: CAPES/PROEX 23038.005848/2018-31
dc.format.extent1677-1682
dc.identifierhttp://dx.doi.org/10.1007/s00705-019-04214-z
dc.identifier.citationArchives of Virology, v. 164, n. 6, p. 1677-1682, 2019.
dc.identifier.doi10.1007/s00705-019-04214-z
dc.identifier.issn0304-8608
dc.identifier.lattes7179273060624761
dc.identifier.scopus2-s2.0-85064200276
dc.identifier.urihttp://hdl.handle.net/11449/188961
dc.language.isoeng
dc.relation.ispartofArchives of Virology
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.titleCell-line-dependent crystal morphology and sublocalization of the Thyrinteina arnobia cypovirus polyhedrin expressed from a recombinant baculovirusen
dc.typeArtigo
unesp.author.lattes7179273060624761
unesp.author.lattes7353607022049208[6]
unesp.author.orcid0000-0002-6292-7990[2]
unesp.author.orcid0000-0001-9875-4158[6]
unesp.departmentProteção Vegetal - FCApt
unesp.departmentBiologia - FCAVpt

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Botucatu - FCA - Faculdade de Ciências Agronômicas
Jaboticabal - FCAV - Faculdade de Ciências Agrárias e Veterinárias