Leukotoxic activity of Actinobacillus actinomycetemcomitans isolated from Brazilian periodontal patients

dc.contributor.authorGuazeli-Amin, V. H.
dc.contributor.authorAvila-Campos, M. J.
dc.contributor.authorBarbosa, D. S.
dc.contributor.authorGaetti-Jardim, E.
dc.contributor.authorTrevisan, W.
dc.contributor.authorItano, E. N.
dc.contributor.institutionUniversidade Estadual de Londrina (UEL)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionState Univ Londrina
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:22:16Z
dc.date.available2014-05-20T15:22:16Z
dc.date.issued2000-12-01
dc.description.abstractThe leukotoxic activity of 31 Actinobacillus actinomycetemcomitans isolates from Brazilian periodontal patients [nine from Localized Juvenile Periodontitis (LJP) patients, 22 from patients with AIDS-associated Necrotizing Ulcerative Periodontitis (AIDS/NUP)], and from the reference strain A. actinomycetemcomitans ATCC43718, were analyses for their cytotoxicity on human monocytes. A cytotoxicity inhibitory assay of the isolate P35 and the reference strain ATCC 43718 with sera from ten LJP patients and ten healthy subjects was also performed and leukotoxin reactivity was evaluated with serum from rabbits immune to leukotoxin from A. actinomycetemcomitans ATCC 43718. The cytotoxicity results were not statistically different among groups of A. actinomycetemcomitans isolates from LJP and AIDS/NUP patients, but the individual analysis of each isolate showed two isolates (P24 and P35) from LJP patients with high leukotoxic activity (P<0.05). Also, a high leucotoxic inhibitory effect with LJP patients' sera compared with healthy subjects with sonic extract from isolate P35 (P<0.05) and the reactivity of rabbit antiserum to leukotoxin were observed. Both leukotoxic and non-leukotoxic activity is more frequent in PJL than AIDS/NUP patients. Even though A. actinomycetemcomitans exhibits leukotoxic activity, there is an immune response to the leucotoxin in LJP patients. (C) 2000 Academic Press.en
dc.description.affiliationUniv Estadual Londrina, CCB, Dept Ciências Patol, BR-86051970 Londrina, PR, Brazil
dc.description.affiliationUniv São Paulo, Dept Microbiol, São Paulo, Brazil
dc.description.affiliationState Univ Londrina, CCS, Dept Clin Pathol & Deontol, Londrina, PR, Brazil
dc.description.affiliationState Univ São Paulo, Dept Pathol, Aracatuba, SP, Brazil
dc.description.affiliationState Univ Londrina, Dept Oral Med & Pediat Odontol, Londrina, PR, Brazil
dc.description.affiliationUnespState Univ São Paulo, Dept Pathol, Aracatuba, SP, Brazil
dc.format.extent341-346
dc.identifierhttp://dx.doi.org/10.1006/anae.2000.0357
dc.identifier.citationAnaerobe. London: Academic Press Ltd, v. 6, n. 6, p. 341-346, 2000.
dc.identifier.doi10.1006/anae.2000.0357
dc.identifier.issn1075-9964
dc.identifier.urihttp://hdl.handle.net/11449/33272
dc.identifier.wosWOS:000166350000004
dc.language.isoeng
dc.publisherAcademic Press Ltd
dc.relation.ispartofAnaerobe
dc.relation.ispartofjcr2.742
dc.relation.ispartofsjr1,144
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectActinobacillus actinomycetemcomitanspt
dc.subjectleukotoxic activitypt
dc.subjectperiodontitispt
dc.subjectneutralizationpt
dc.subjectAIDSpt
dc.titleLeukotoxic activity of Actinobacillus actinomycetemcomitans isolated from Brazilian periodontal patientsen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderAcademic Press Ltd
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araçatubapt
unesp.departmentPatologia e Propedêutica Clínica - FOApt

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