Periapical lesions decrease Akt serine phosphorylation and plasma membrane GLUT4 content in rat skeletal muscle

dc.contributor.authorPereira, Renato Felipe [UNESP]
dc.contributor.authorde Oliveira da Mota, Max Sander [UNESP]
dc.contributor.authorde Lima Coutinho Mattera, Maria Sara [UNESP]
dc.contributor.authorTsosura, Thaís Verônica Saori [UNESP]
dc.contributor.authorChiba, Fernando Yamamoto [UNESP]
dc.contributor.authorGarbin, Cléa Adas Saliba [UNESP]
dc.contributor.authorErvolino, Edilson [UNESP]
dc.contributor.authorCintra, Luciano Tavares Angelo [UNESP]
dc.contributor.authorOkamoto, Maristela Mitiko
dc.contributor.authorMachado, Ubiratan Fabres
dc.contributor.authorSumida, Doris Hissako [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2018-12-11T16:59:19Z
dc.date.available2018-12-11T16:59:19Z
dc.date.issued2016-09-01
dc.description.abstractObjectives: Periapical lesion (PL) promotes insulin resistance; however, the mechanisms underlying this alteration are not fully understood. Therefore, in this study, we aimed to evaluate the Akt serine phosphorylation status and GLUT4 expression levels in the gastrocnemius muscle (GM) of rats with PL. Materials and methods: Male Wistar rats (n = 42) were distributed equally into control (CN) and PL groups. The pulpal tissue of the PL group rats was exposed to the oral environment for 30 days. Thereafter, glucose and insulin levels were assessed, followed by homeostasis model assessment of insulin resistance (HOMA-IR). The Akt serine phosphorylation and GLUT4 levels of microsomal (M) and plasma membrane (PM) fractions were evaluated by western blotting and analyzed statistically. Results: Compared to CN group rats, PL group rats had lower insulin sensitivity (as observed by HOMA-IR), lower Akt serine phosphorylation status after insulin stimulus, and lower GLUT4 levels in the PM fraction. However, the M fraction in the PL group did not differ significantly from that of the CN group. Conclusions: PL decreases insulin sensitivity, Akt phosphorylation, and PM GLUT4 content. Clinical relevance: The present study indicates that preventing endodontic disease can thwart insulin resistance.en
dc.description.affiliationPrograma de Pós-graduação Multicêntrico em Ciências Fisiológicas-SBFis Department of Basic Sciences Araçatuba Dental School Univ Estadual Paulista (UNESP), Rod. Marechal Rondom, km 527/528
dc.description.affiliationDepartment of Child and Social Dentistry Araçatuba Dental School Univ Estadual Paulista (UNESP)
dc.description.affiliationEndodontics Araçatuba Dental School Univ Estadual Paulista (UNESP)
dc.description.affiliationDepartment of Physiology and Biophysics São Paulo Institute of Biomedical Sciences University of São Paulo (USP), Av. Prof. Lineu Prestes, 2415
dc.description.affiliationUnespPrograma de Pós-graduação Multicêntrico em Ciências Fisiológicas-SBFis Department of Basic Sciences Araçatuba Dental School Univ Estadual Paulista (UNESP), Rod. Marechal Rondom, km 527/528
dc.description.affiliationUnespDepartment of Child and Social Dentistry Araçatuba Dental School Univ Estadual Paulista (UNESP)
dc.description.affiliationUnespEndodontics Araçatuba Dental School Univ Estadual Paulista (UNESP)
dc.format.extent1625-1630
dc.identifierhttp://dx.doi.org/10.1007/s00784-015-1664-4
dc.identifier.citationClinical Oral Investigations, v. 20, n. 7, p. 1625-1630, 2016.
dc.identifier.doi10.1007/s00784-015-1664-4
dc.identifier.file2-s2.0-84948171836.pdf
dc.identifier.issn1436-3771
dc.identifier.issn1432-6981
dc.identifier.lattes4408095517346846
dc.identifier.lattes9235743081667362
dc.identifier.lattes4419158525709686
dc.identifier.orcid0000-0003-4859-0583
dc.identifier.orcid0000-0003-4859-0583
dc.identifier.orcid0000-0001-5069-8812
dc.identifier.scopus2-s2.0-84948171836
dc.identifier.urihttp://hdl.handle.net/11449/172237
dc.language.isoeng
dc.relation.ispartofClinical Oral Investigations
dc.relation.ispartofsjr0,986
dc.relation.ispartofsjr0,986
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectDiabetes mellitus
dc.subjectGlucose transporter type 4
dc.subjectInsulin resistance
dc.subjectPeriapical lesions
dc.titlePeriapical lesions decrease Akt serine phosphorylation and plasma membrane GLUT4 content in rat skeletal muscleen
dc.typeArtigo
unesp.author.lattes4408095517346846[7]
unesp.author.lattes9235743081667362
unesp.author.lattes4419158525709686[6]
unesp.author.orcid0000-0003-4859-0583[7]
unesp.author.orcid0000-0001-5069-8812[6]

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