Increased Viability of Odontoblast-Like Cells Subjected to Low-Level Laser Irradiation
dc.contributor.author | Oliveira, C. F. [UNESP] | |
dc.contributor.author | Basso, F. G. | |
dc.contributor.author | Lins, E. C. | |
dc.contributor.author | Kurachi, C. | |
dc.contributor.author | Hebling, Josimeri [UNESP] | |
dc.contributor.author | Bagnato, V. S. | |
dc.contributor.author | de Souza Costa, C. A. [UNESP] | |
dc.contributor.institution | Universidade Estadual Paulista (Unesp) | |
dc.contributor.institution | Universidade Estadual de Campinas (UNICAMP) | |
dc.contributor.institution | Universidade de São Paulo (USP) | |
dc.date.accessioned | 2013-09-30T18:31:29Z | |
dc.date.accessioned | 2014-05-20T13:44:57Z | |
dc.date.available | 2013-09-30T18:31:29Z | |
dc.date.available | 2014-05-20T13:44:57Z | |
dc.date.issued | 2010-07-01 | |
dc.description.abstract | Studies have shown that the increase of cell metabolism depends on the low level laser therapy (LLLT) parameters used to irradiate the cells. However, the optimal laser dose to up-regulate pulp cell activity remains unknown. Consequently, the aim of this study was to evaluate the metabolic response of odontoblast-like cells (MDPC-23) exposed to different LLLT doses. Cells at 20000 cells/cm(2) were seeded in 24-well plates using plain culture medium (DMEM) and were incubated in a humidified incubator with 5% CO2 at 37 degrees C. After 24 h, the culture medium was replaced by fresh DMEM supplemented with 5% (stress by nutritional deficit) or 10% fetal bovine serum (FBS). The cells were exposed to different laser doses from a near infrared diode laser prototype designed to provide a uniform irradiation of the wells. The experimental groups were: G1: 1.5 J/cm(2) + 5% FBS; G2: 1.5 J/cm(2) + 10% FBS; G3: 5 J/cm(2) + 5% FBS; G4: 5 J/cm(2) + 10% FBS; G5: 19 J/cm(2) + 5% FBS; G6: 19 J/cm(2) + 10% FBS. LLLT was performed in 3 consecutive irradiation cycles with a 24-hour interval. Non-irradiated cells cultured in DMEM supplemented with either 5 or 10% FBS served as control groups. The analysis of the metabolic response was performed by the MTT assay 3 h after the last irradiation. G1 presented an increase in SDH enzyme activity and differed significantly (Mann-Whitney test, p < 0.05) from the other groups. Analysis by scanning electron microscopy showed normal cell morphology in all groups. Under the tested conditions, LLLT stimulated the metabolic activity of MDPC-23 cultured in DMEM supplemented with 5% FBS and exposed to a laser dose of 1.5 J/cm(2). These findings are relevant for further studies on the action of near infrared lasers on cells with odontoblast phenotype. | en |
dc.description.affiliation | UNESP Univ Estadual Paulista, Araraquara Sch Dent, BR-14801903 Araraquara, SP, Brazil | |
dc.description.affiliation | UNICAMP Univ Campinas, Piracicaba Sch Dent, BR-13414903 Piracicaba, SP, Brazil | |
dc.description.affiliation | Univ São Paulo, Phys Inst São Carlos, BR-13560970 São Carlos, SP, Brazil | |
dc.description.affiliationUnesp | UNESP Univ Estadual Paulista, Araraquara Sch Dent, BR-14801903 Araraquara, SP, Brazil | |
dc.description.sponsorship | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | |
dc.description.sponsorship | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | |
dc.description.sponsorshipId | FAPESP: 07/50646-3 | |
dc.description.sponsorshipId | FAPESP: 08/54785-0 | |
dc.description.sponsorshipId | CNPq: 476137/2006 | |
dc.description.sponsorshipId | CNPq: 301029/2007-5 | |
dc.format.extent | 1659-1666 | |
dc.identifier | http://dx.doi.org/10.1134/S1054660X10130153 | |
dc.identifier.citation | Laser Physics. New York: Maik Nauka/interperiodica/springer, v. 20, n. 7, p. 1659-1666, 2010. | |
dc.identifier.doi | 10.1134/S1054660X10130153 | |
dc.identifier.issn | 1054-660X | |
dc.identifier.lattes | 4517484241515548 | |
dc.identifier.uri | http://hdl.handle.net/11449/15779 | |
dc.identifier.wos | WOS:000280662100022 | |
dc.language.iso | eng | |
dc.publisher | Maik Nauka/interperiodica/springer | |
dc.relation.ispartof | Laser Physics | |
dc.relation.ispartofjcr | 1.158 | |
dc.rights.accessRights | Acesso restrito | |
dc.source | Web of Science | |
dc.title | Increased Viability of Odontoblast-Like Cells Subjected to Low-Level Laser Irradiation | en |
dc.type | Artigo | |
dcterms.license | http://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0 | |
dcterms.rightsHolder | Maik Nauka/interperiodica/springer | |
unesp.author.lattes | 4517484241515548[7] | |
unesp.author.orcid | 0000-0002-7455-6867[7] | |
unesp.author.orcid | 0000-0002-7967-6163[3] | |
unesp.campus | Universidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquara | pt |
unesp.department | Clínica Infantil - FOAR | pt |
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