Increased Viability of Odontoblast-Like Cells Subjected to Low-Level Laser Irradiation

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dc.contributor.authorOliveira, C. F. [UNESP]
dc.contributor.authorBasso, F. G.
dc.contributor.authorLins, E. C.
dc.contributor.authorKurachi, C.
dc.contributor.authorHebling, Josimeri [UNESP]
dc.contributor.authorBagnato, V. S.
dc.contributor.authorde Souza Costa, C. A. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2013-09-30T18:31:29Z
dc.date.accessioned2014-05-20T13:44:57Z
dc.date.available2013-09-30T18:31:29Z
dc.date.available2014-05-20T13:44:57Z
dc.date.issued2010-07-01
dc.description.abstractStudies have shown that the increase of cell metabolism depends on the low level laser therapy (LLLT) parameters used to irradiate the cells. However, the optimal laser dose to up-regulate pulp cell activity remains unknown. Consequently, the aim of this study was to evaluate the metabolic response of odontoblast-like cells (MDPC-23) exposed to different LLLT doses. Cells at 20000 cells/cm(2) were seeded in 24-well plates using plain culture medium (DMEM) and were incubated in a humidified incubator with 5% CO2 at 37 degrees C. After 24 h, the culture medium was replaced by fresh DMEM supplemented with 5% (stress by nutritional deficit) or 10% fetal bovine serum (FBS). The cells were exposed to different laser doses from a near infrared diode laser prototype designed to provide a uniform irradiation of the wells. The experimental groups were: G1: 1.5 J/cm(2) + 5% FBS; G2: 1.5 J/cm(2) + 10% FBS; G3: 5 J/cm(2) + 5% FBS; G4: 5 J/cm(2) + 10% FBS; G5: 19 J/cm(2) + 5% FBS; G6: 19 J/cm(2) + 10% FBS. LLLT was performed in 3 consecutive irradiation cycles with a 24-hour interval. Non-irradiated cells cultured in DMEM supplemented with either 5 or 10% FBS served as control groups. The analysis of the metabolic response was performed by the MTT assay 3 h after the last irradiation. G1 presented an increase in SDH enzyme activity and differed significantly (Mann-Whitney test, p < 0.05) from the other groups. Analysis by scanning electron microscopy showed normal cell morphology in all groups. Under the tested conditions, LLLT stimulated the metabolic activity of MDPC-23 cultured in DMEM supplemented with 5% FBS and exposed to a laser dose of 1.5 J/cm(2). These findings are relevant for further studies on the action of near infrared lasers on cells with odontoblast phenotype.en
dc.description.affiliationUNESP Univ Estadual Paulista, Araraquara Sch Dent, BR-14801903 Araraquara, SP, Brazil
dc.description.affiliationUNICAMP Univ Campinas, Piracicaba Sch Dent, BR-13414903 Piracicaba, SP, Brazil
dc.description.affiliationUniv São Paulo, Phys Inst São Carlos, BR-13560970 São Carlos, SP, Brazil
dc.description.affiliationUnespUNESP Univ Estadual Paulista, Araraquara Sch Dent, BR-14801903 Araraquara, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdFAPESP: 07/50646-3
dc.description.sponsorshipIdFAPESP: 08/54785-0
dc.description.sponsorshipIdCNPq: 476137/2006
dc.description.sponsorshipIdCNPq: 301029/2007-5
dc.format.extent1659-1666
dc.identifierhttp://dx.doi.org/10.1134/S1054660X10130153
dc.identifier.citationLaser Physics. New York: Maik Nauka/interperiodica/springer, v. 20, n. 7, p. 1659-1666, 2010.
dc.identifier.doi10.1134/S1054660X10130153
dc.identifier.issn1054-660X
dc.identifier.lattes4517484241515548
dc.identifier.urihttp://hdl.handle.net/11449/15779
dc.identifier.wosWOS:000280662100022
dc.language.isoeng
dc.publisherMaik Nauka/interperiodica/springer
dc.relation.ispartofLaser Physics
dc.relation.ispartofjcr1.158
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.titleIncreased Viability of Odontoblast-Like Cells Subjected to Low-Level Laser Irradiationen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderMaik Nauka/interperiodica/springer
unesp.author.lattes4517484241515548[7]
unesp.author.orcid0000-0002-7455-6867[7]
unesp.author.orcid0000-0002-7967-6163[3]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt

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