Determination of zinc in rice grains using DTZ staining and ImageJ software

dc.contributor.authorDuarte, Romulo F.
dc.contributor.authorProm-u-Thai, Chanakan
dc.contributor.authorAmaral, Douglas C.
dc.contributor.authorFaquin, Valdemar
dc.contributor.authorGuilherme, Luiz R. G.
dc.contributor.authorReis, Andre R. [UNESP]
dc.contributor.authorAlves, Eduardo
dc.contributor.institutionChiang Mai Univ
dc.contributor.institutionUniversidade Federal de Lavras (UFLA)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.description.abstractDithizone (DTZ) staining is a rapid, simple, and inexpensive method that allows the histochemical localization of Zn. In this study, we evaluated genotypic variation in Zn concentration in brown rice grown in the field using atomic absorption spectrophotometer (AAS) as DTZ staining quantified using ImageJ software. We used dehusked grains from upland rice accessions widely cultivated in Brazil. The DTZ staining showed that the concentration of Zn varied within and between rice grains. The staining intensity index (Y) provided differences in localization of Zn across the grain regions. Zn concentration in brown rice varied in multiple regression analysis, showing major differences in index weighted by stained area of each region (YAW) for the embryo, endosperm, and aleurone, especially in the endosperm and aleurone regions due to a large portion of the area in the kernel. The total YAW among rice accessions was positively correlated with Zn concentration in grains by chemical analysis. The DTZ staining associated with imageJ software is a promising to estimate Zn concentration in different grain tissues. Thus,. this method may be useful for rapid screening of rice germplasms. (C) 2015 Elsevier Ltd. All rights reserved.en
dc.description.affiliationChiang Mai Univ, Fac Agr, Dept Plant Sci & Nat Resources, Div Agron, Chiang Mai 50200, Thailand
dc.description.affiliationUniv Fed Lavras, Dept Soil Sci, POB 3037, BR-37200000 Lavras, MG, Brazil
dc.description.affiliationChiang Mai Univ, Lanna Rice Res Ctr, Chiang Mai 50200, Thailand
dc.description.affiliationSao Paulo State Univ, Fac Sci & Engn, Biosyst Engn, BR-17602496 Tupa, SP, Brazil
dc.description.affiliationUniv Fed Lavras, Dept Plant Pathol, POB 3037, BR-37200000 Lavras, MG, Brazil
dc.description.affiliationUnespSao Paulo State Univ, Fac Sci & Engn, Biosyst Engn, BR-17602496 Tupa, SP, Brazil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)
dc.description.sponsorshipNational Research University Programme of Thailand's Commission on Higher Education
dc.identifier.citationJournal Of Cereal Science. London: Academic Press Ltd- Elsevier Science Ltd, v. 68, p. 53-58, 2016.
dc.publisherElsevier B.V.
dc.relation.ispartofJournal Of Cereal Science
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectOryza sativa L.
dc.subjectZinc deficiency
dc.subjectStaining intensity
dc.titleDetermination of zinc in rice grains using DTZ staining and ImageJ softwareen
dcterms.rightsHolderElsevier B.V.[6][6]


Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
734.16 KB
Adobe Portable Document Format