Prevalence and genetic characterization of Dientamoeba fragilis in asymptomatic children attending daycare centers

dc.contributor.authorOliveira-Arbex, Ana Paula [UNESP]
dc.contributor.authorDavid, Erica Boarato [UNESP]
dc.contributor.authorCaccio, Simone Mario
dc.contributor.authorBranco da Fonseca, Catia Regina [UNESP]
dc.contributor.authorMartin, Joelma Goncalves [UNESP]
dc.contributor.authorKurokawa, Cilmery Suemi [UNESP]
dc.contributor.authorTosini, Fabio
dc.contributor.authorSouza-Neto, Jayme Augusto [UNESP]
dc.contributor.authorGuimaraes, Semiramis [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionFac Integradas Bauru
dc.contributor.institutionUNISAGRAD
dc.contributor.institutionIst Super Sanita
dc.date.accessioned2021-06-25T15:05:24Z
dc.date.available2021-06-25T15:05:24Z
dc.date.issued2021-01-01
dc.description.abstractIn order to provide additional data on the prevalence and genetic diversity of Dientantoeba fragilis in human populations, we conducted a study in children from low-income communities in Sao Paulo State. Brazil. Fecal samples from daycare center attendees up to 6 years old (n=156) and staff members (n=18) were submitted to PCR and sequencing of D. fragilis as well as to microscopic examination for the presence of other intestinal parasites. All children assessed were asymptomatic and 10.3% (16/156) were positive for D. fragilis. No worker was found to be positive. An association between Dientamoeba and coinfection with other intestinal parasites was observed. Concerning the genetic diversity, 14 and only two isolates were genotype 1 and genotype 2, respectively. Our findings outline interesting aspects: (1) asymptomatic children as carriers of Dientamoeba in communities in which environmental conditions ensure parasite transmission and. (2) association between Dientamoeba infection in young children and coinfection with other enteric parasites, reinforcing its transmission via the fecal-oral route.en
dc.description.affiliationUniv Estadual Paulista, Inst Biociencias, Dept Parasitol, Caixa Postal 510, BR-18618970 Botucatu, SP, Brazil
dc.description.affiliationFac Integradas Bauru, Bauru, SP, Brazil
dc.description.affiliationUNISAGRAD, Dept Ciencias Saude, Bauru, SP, Brazil
dc.description.affiliationIst Super Sanita, Dipartimento Malattie Infett Parassitarie & Immun, Rome, Italy
dc.description.affiliationUniv Estadual Paulista, Fac Med, Dept Pediat, Botucatu, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Fac Ciencias Agron, Dept Bioproc & Biotecnol, Botucatu, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Inst Biociencias, Dept Parasitol, Caixa Postal 510, BR-18618970 Botucatu, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Med, Dept Pediat, Botucatu, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Ciencias Agron, Dept Bioproc & Biotecnol, Botucatu, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2015/21254-6
dc.format.extent6
dc.identifierhttp://dx.doi.org/10.1590/S1678-9946202163039
dc.identifier.citationRevista Do Instituto De Medicina Tropical De Sao Paulo. Sao Paulo: Inst Medicina Tropical Sao Paulo, v. 63, 6 p., 2021.
dc.identifier.doi10.1590/S1678-9946202163039
dc.identifier.fileS0036-46652021000100603.pdf
dc.identifier.issn0036-4665
dc.identifier.lattes8510423269540465
dc.identifier.orcid0000-0003-1380-7527
dc.identifier.scieloS0036-46652021000100603
dc.identifier.urihttp://hdl.handle.net/11449/210341
dc.identifier.wosWOS:000651644300001
dc.language.isoeng
dc.publisherInst Medicina Tropical Sao Paulo
dc.relation.ispartofRevista Do Instituto De Medicina Tropical De Sao Paulo
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectDientamoeba fragilis
dc.subjectPrevalence
dc.subjectChildren
dc.subjectPCR
dc.subjectGenotypes
dc.titlePrevalence and genetic characterization of Dientamoeba fragilis in asymptomatic children attending daycare centersen
dc.typeArtigo
dcterms.rightsHolderInst Medicina Tropical Sao Paulo
unesp.author.lattes8510423269540465[6]
unesp.author.orcid0000-0003-1120-5725[7]
unesp.author.orcid0000-0003-1380-7527[6]

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