Ultraviolet light treatment of titanium suppresses human oral bacterial attachment and biofilm formation: A short-term in vitro study

dc.contributor.authorIshijima, Manabu
dc.contributor.authorde Avila, Erica Dorigatti [UNESP]
dc.contributor.authorNakhaei, Kourosh
dc.contributor.authorShi, Wenyuan
dc.contributor.authorLux, Renate
dc.contributor.authorOgawa, Takahiro
dc.contributor.institutionUCLA School of Dentistry
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionThe Forsyth Institute
dc.date.accessioned2020-12-12T01:39:42Z
dc.date.available2020-12-12T01:39:42Z
dc.date.issued2019-01-01
dc.description.abstractAntibacterial dental implants and related prosthetic components could help to reduce infection and prevent peri-implantitis. The purpose of this study was to determine the effect of ultraviolet (UV) light treatment of titanium on biofilm formation of human oral bacteria. Materials and Methods: Machineprepared commercially pure titanium disks were treated with UV light for 12 minutes. Human oral bacteria were seeded onto untreated and UV-treated disks. Early bacterial attachment to titanium was assessed at 12 hours. Surface topography of initial biofilms was evaluated by 3D scanning electron microscopy at 24 hours. The quantity and morphology of subsequent colony development and biofilm formation were examined by confocal laser scanning microscopy for up to 7 days. Results: Throughout the time course, significantly fewer bacterial cells attached to UV-treated titanium surfaces compared with untreated ones. While biofilm developed rapidly to a final thickness of approximately 16 μm by day 3 on untreated titanium, on UV-treated surfaces it remained below 8 μm, even at day 7. Similarly, UV treatment resulted in 70% less exopolysaccharide (EPS) volume than on untreated surfaces at day 7. This is consistent with the finding that EPS production per cell was significantly lower on UV-treated surfaces. Untreated titanium surfaces covered with biofilm were fivefold rougher than the original machined surface, while UV-treated surfaces remained twofold rougher due to significantly less biofilm formation. Conclusion: UV treatment of titanium surfaces significantly reduces attachment of human oral bacteria and subsequent biofilm formation as well as EPS production for at least 7 days. UV treatment prevented the escalation of surface colonization, mitigating an unfavorable bacteriophilic cascade and environmental trigger for biofilm formation.en
dc.description.affiliationWeintraub Center for Reconstructive Biotechnology Division of Advanced Prosthodontics UCLA School of Dentistry
dc.description.affiliationDivision of Oral Biology and Medicine UCLA School of Dentistry
dc.description.affiliationDepartment of Dental Materials and Prosthodontics School of Dentistry at Araraquara São Paulo State University - UNESP
dc.description.affiliationThe Forsyth Institute
dc.description.affiliationDivision of Constitutive and Regenerative Sciences UCLA School of Dentistry
dc.description.affiliationUnespDepartment of Dental Materials and Prosthodontics School of Dentistry at Araraquara São Paulo State University - UNESP
dc.format.extent1105-1113
dc.identifierhttp://dx.doi.org/10.11607/jomi.7444
dc.identifier.citationInternational Journal of Oral and Maxillofacial Implants, v. 34, n. 5, p. 1105-1113, 2019.
dc.identifier.doi10.11607/jomi.7444
dc.identifier.issn1942-4434
dc.identifier.issn0882-2786
dc.identifier.scopus2-s2.0-85072508834
dc.identifier.urihttp://hdl.handle.net/11449/199438
dc.language.isoeng
dc.relation.ispartofInternational Journal of Oral and Maxillofacial Implants
dc.sourceScopus
dc.subjectDental implants
dc.subjectPeri-implantitis
dc.subjectTitanium
dc.subjectUltraviolet
dc.titleUltraviolet light treatment of titanium suppresses human oral bacterial attachment and biofilm formation: A short-term in vitro studyen
dc.typeArtigo
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt
unesp.departmentMateriais Odontológicos e Prótese - FOARpt

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