Detecção de Paracoccidioides sp. em amostras ambientais aerossóis

Carregando...
Imagem de Miniatura

Data

2012-02-24

Autores

Arantes, Thales Domingos [UNESP]

Título da Revista

ISSN da Revista

Título de Volume

Editor

Universidade Estadual Paulista (Unesp)

Resumo

Taking into account that paracoccidioidomycosis infection occurs by inhalation of the asexual conidia produced by Paracoccidioides sp. in its saprobic phase, this work presents the collection of aerosol samples as an option for environmental detection of this pathogen, by positioning a cyclonic air sampler at the entrance of armadillo burrows. Detection strategies included: direct culture, extinction technique culture and Nested PCR of the ITS1-5.8S-ITS2 region, as well as the evaluation of one armadillo (D. novemcinctus) as positive control for the studied area. Although the pathogen could not be isolated by the culturing strategies, the aerosol sampling associated with molecular detection by Nested PCR proved the best method for detecting Paracoccidioides sp. in the environment. Most of the ITS sequences obtained herein presented high similarity with the homologous sequences of P. lutzii from GenBank database, suggesting that this Paracoccidioides species may be not exclusive to mid-western Brazil as proposed so far

Descrição

Palavras-chave

Paracoccidioides brasiliensis, Paracoccidioidomicose, Fungos patogenicos, Ecologia, Impacto ambiental - Avaliação, Infection by inhalation

Como citar

ARANTES, Thales Domingos. Detecção de Paracoccidioides sp. em amostras ambientais aerossóis. 2012. 65 f. Dissertação (mestrado) - Universidade Estadual Paulista Julio de Mesquita Filho, Instituto de Biociências de Botucatu, 2012.