Hormonal control and target genes of ftz-f1 expression in the honeybee Apis mellifera: a positive loop linking juvenile hormone, ftz-f1, and vitellogenin

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dc.contributor.authorMello, T. R.P.
dc.contributor.authorAleixo, A. C.
dc.contributor.authorPinheiro, D. G. [UNESP]
dc.contributor.authorNunes, F. M.F.
dc.contributor.authorCristino, A. S.
dc.contributor.authorBitondi, M. M.G.
dc.contributor.authorBarchuk, A. R.
dc.contributor.authorSimões, Z. L.P.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)
dc.contributor.institutionThe University of Queensland Diamantina Institute
dc.contributor.institutionUNIFAL-MG
dc.date.accessioned2019-10-06T15:22:45Z
dc.date.available2019-10-06T15:22:45Z
dc.date.issued2019-02-01
dc.description.abstractFtz-f1 is an orphan member of the nuclear hormone receptor superfamily. A 20-hydroxyecdysone pulse allows ftz-f1 gene expression, which then regulates the activity of downstream genes involved in major developmental progression events. In honeybees, the expression of genes like vitellogenin (vg), prophenoloxidase and juvenile hormone-esterase during late pharate-adult development is known to be hormonally controlled in both queens and workers by increasing juvenile hormone (JH) titres in the presence of declining levels of ecdysteroids. Since Ftz-f1 is known for mediating intracellular JH signalling, we hypothesized that ftz-f1 could mediate JH action during the pharate-adult development of honeybees, thus controlling the expression of these genes. Here, we show that ftz-f1 has caste-specific transcription profiles during this developmental period, with a peak coinciding with the increase in JH titre, and that its expression is upregulated by JH and downregulated by ecdysteroids. RNAi-mediated knock down of ftz-f1 showed that the expression of genes essential for adult development (e.g. vg and cuticular genes) depends on ftz-f1 expression. Finally, a double-repressor hypothesis-inspired vg gene knock-down experiment suggests the existence of a positive molecular loop between JH, ftz-f1 and vg.en
dc.description.affiliationDepartamento de Genética Faculdade de Medicina de Ribeirão Preto Universidade de São Paulo
dc.description.affiliationFaculdade de Ciências Agrárias e Veterinárias UNESP – Universidade Estadual Paulista
dc.description.affiliationDepartamento de Genética e Evolução Centro de Ciências Biológicas e da Saúde Universidade Federal de São Carlos
dc.description.affiliationTranslational Research Institute The University of Queensland Diamantina Institute
dc.description.affiliationDepartamento de Biologia Faculdade de Filosofia Ciências e Letras de Ribeirão Preto Universidade de São Paulo
dc.description.affiliationDepartamento de Biologia Celular e do Desenvolvimento Instituto de Ciências Biomédicas Universidade Federal de Alfenas UNIFAL-MG
dc.description.affiliationUnespFaculdade de Ciências Agrárias e Veterinárias UNESP – Universidade Estadual Paulista
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdFAPESP: 2005/03926-5
dc.description.sponsorshipIdFAPESP: 2008/10757-3
dc.description.sponsorshipIdFAPESP: 2011/03171-5
dc.description.sponsorshipIdCNPq: 307426/2014-9
dc.format.extent145-159
dc.identifierhttp://dx.doi.org/10.1111/imb.12541
dc.identifier.citationInsect Molecular Biology, v. 28, n. 1, p. 145-159, 2019.
dc.identifier.doi10.1111/imb.12541
dc.identifier.issn1365-2583
dc.identifier.issn0962-1075
dc.identifier.scopus2-s2.0-85055683303
dc.identifier.urihttp://hdl.handle.net/11449/187011
dc.language.isoeng
dc.relation.ispartofInsect Molecular Biology
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subject20-hydroxyecdysone
dc.subject20E
dc.subjectFushi tarazu transcription factor 1
dc.subjecthoney bee
dc.subjectjuvenile hormone
dc.subjectRNAi
dc.titleHormonal control and target genes of ftz-f1 expression in the honeybee Apis mellifera: a positive loop linking juvenile hormone, ftz-f1, and vitellogeninen
dc.typeArtigo

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