Avaliação longitudinal da resposta immune humoral e diversidade de antígenos de superfície de merozoítos em bezerros naturalmente infectados com Babesia bovis, em São Paulo, Brasil

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dc.contributor.authorMatos, Carlos António [UNESP]
dc.contributor.authorGonçalves, Luiz Ricardo [UNESP]
dc.contributor.authorAlvarez, Dasiel Obregón
dc.contributor.authorFreschi, Carla Roberta [UNESP]
dc.contributor.authorda Silva, Jenevaldo Barbosa [UNESP]
dc.contributor.authorVal-Moraes, Silvana Pompeia [UNESP]
dc.contributor.authorMendes, Natalia Serra [UNESP]
dc.contributor.authorAndré, Marcos Rogério [UNESP]
dc.contributor.authorMachado, Rosangela Zacarias [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionDirecção de Ciências Animais
dc.contributor.institutionUniversidad Agraria de La Habana
dc.date.accessioned2018-12-11T17:35:03Z
dc.date.available2018-12-11T17:35:03Z
dc.date.issued2017-10-01
dc.description.abstractBabesiosis is an economically important infectious disease affecting cattle worldwide. In order to longitudinally evaluate the humoral immune response against Babesia bovis and the merozoite surface antigen diversity of B. bovis among naturally infected calves in Taiaçu, Brazil, serum and DNA samples from 15 calves were obtained quarterly, from their birth to 12 months of age. Anti-B. bovis IgG antibodies were detected by means of the indirect fluorescent antibody test (IFAT) and enzyme-linked immunosorbent assay (ELISA). The polymerase chain reaction (PCR) was used to investigate the genetic diversity of B. bovis, based on the genes that encode merozoite surface antigens (MSA-1, MSA-2b and MSA‑2c). The serological results demonstrated that up to six months of age, all the calves developed active immunity against B. bovis. Among the 75 DNA samples evaluated, 2, 4 and 5 sequences of the genes msa-1, msa-2b and msa-2c were obtained. The present study demonstrated that the msa-1 and msa-2b genes sequences amplified from blood DNA of calves positive to B. bovis from Taiaçu were genetically distinct, and that msa-2c was conserved. All animals were serologically positive to ELISA and IFAT, which used full repertoire of parasite antigens in despite of the genetic diversity of MSAs.en
dc.description.affiliationLaboratório de Imunoparasitologia Departamento de Patologia Veterinária Universidade Estadual Paulista – UNESP
dc.description.affiliationDirecção de Ciências Animais
dc.description.affiliationUniversidad Agraria de La Habana
dc.description.affiliationUnespLaboratório de Imunoparasitologia Departamento de Patologia Veterinária Universidade Estadual Paulista – UNESP
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent479-490
dc.identifierhttp://dx.doi.org/10.1590/S1984-29612017069
dc.identifier.citationRevista Brasileira de Parasitologia Veterinaria, v. 26, n. 4, p. 479-490, 2017.
dc.identifier.doi10.1590/S1984-29612017069
dc.identifier.fileS1984-29612017000400479.pdf
dc.identifier.issn0103-846X
dc.identifier.lattes9139899895580513
dc.identifier.scieloS1984-29612017000400479
dc.identifier.scopus2-s2.0-85036562592
dc.identifier.urihttp://hdl.handle.net/11449/179407
dc.language.isoeng
dc.relation.ispartofRevista Brasileira de Parasitologia Veterinaria
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectBovine babesiosis
dc.subjectGenetic diversity
dc.subjectMSA
dc.subjectSerology
dc.titleAvaliação longitudinal da resposta immune humoral e diversidade de antígenos de superfície de merozoítos em bezerros naturalmente infectados com Babesia bovis, em São Paulo, Brasilpt
dc.title.alternativeLongitudinal evaluation of humoral immune response and merozoite surface antigen diversity in calves naturally infected with babesia bovis, in São Paulo, Brazilen
dc.typeArtigo
unesp.author.lattes9139899895580513

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