Expanding the Functionality of an Autoinduction Device for Repression of Gene Expression in Bacillus subtilis
dc.contributor.author | Silva, Bruna F. [UNESP] | |
dc.contributor.author | Corrêa, Graciely G. [UNESP] | |
dc.contributor.author | Zocca, Vitória F. B. [UNESP] | |
dc.contributor.author | Picheli, Flavio P. [UNESP] | |
dc.contributor.author | Lins, Milca R. C. R. | |
dc.contributor.author | Pedrolli, Danielle B. [UNESP] | |
dc.contributor.institution | Universidade Estadual Paulista (UNESP) | |
dc.contributor.institution | Universidade Federal do ABC (UFABC) | |
dc.date.accessioned | 2023-07-29T12:46:12Z | |
dc.date.available | 2023-07-29T12:46:12Z | |
dc.date.issued | 2023-01-01 | |
dc.description.abstract | Autonomous control of gene expression through engineered quorum-sensing processes is broadly applicable to biosynthetic pathways, including simultaneous control of different genes. It is also a powerful tool for balancing growth and production. We had previously engineered a modular autoinduction device for the control of gene expression in B. subtilis. Now, we expand its functionality to repress gene expression autonomously. The engineered R8 promoter responds to AHL accumulation in the culture medium. In a riboflavin-producing strain, the AHL-Lux complex exerts 5-fold repression on the R8-driven expression of the flavokinase/FAD synthetase gene ribC, resulting in a higher titer of the vitamin. We engineered a strain able to autonomously induce and repress different genes simultaneously, demonstrating the potential of the device for use in metabolic engineering. | en |
dc.description.affiliation | Department of Bioprocess Engineering and Biotechnology School of Pharmaceutical Sciences Universidade Estadual Paulista (UNESP), Rodovia Araraquara-Jau km 1 | |
dc.description.affiliation | Center for Natural and Human Sciences Federal University of ABC (UFABC), Campus Santo André, Avenida dos Estados 5001 | |
dc.description.affiliationUnesp | Department of Bioprocess Engineering and Biotechnology School of Pharmaceutical Sciences Universidade Estadual Paulista (UNESP), Rodovia Araraquara-Jau km 1 | |
dc.identifier | http://dx.doi.org/10.3390/ijms24010084 | |
dc.identifier.citation | International Journal of Molecular Sciences, v. 24, n. 1, 2023. | |
dc.identifier.doi | 10.3390/ijms24010084 | |
dc.identifier.issn | 1422-0067 | |
dc.identifier.issn | 1661-6596 | |
dc.identifier.scopus | 2-s2.0-85146023104 | |
dc.identifier.uri | http://hdl.handle.net/11449/246629 | |
dc.language.iso | eng | |
dc.relation.ispartof | International Journal of Molecular Sciences | |
dc.source | Scopus | |
dc.subject | autoinduction | |
dc.subject | autonomous control | |
dc.subject | Bacillus subtilis | |
dc.subject | dynamic regulation | |
dc.subject | quorum-sensing | |
dc.subject | riboflavin | |
dc.title | Expanding the Functionality of an Autoinduction Device for Repression of Gene Expression in Bacillus subtilis | en |
dc.type | Artigo | |
unesp.author.orcid | 0000-0002-0005-096X[2] | |
unesp.author.orcid | 0000-0002-3034-6497[6] |