Expanding the Functionality of an Autoinduction Device for Repression of Gene Expression in Bacillus subtilis

dc.contributor.authorSilva, Bruna F. [UNESP]
dc.contributor.authorCorrêa, Graciely G. [UNESP]
dc.contributor.authorZocca, Vitória F. B. [UNESP]
dc.contributor.authorPicheli, Flavio P. [UNESP]
dc.contributor.authorLins, Milca R. C. R.
dc.contributor.authorPedrolli, Danielle B. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade Federal do ABC (UFABC)
dc.date.accessioned2023-07-29T12:46:12Z
dc.date.available2023-07-29T12:46:12Z
dc.date.issued2023-01-01
dc.description.abstractAutonomous control of gene expression through engineered quorum-sensing processes is broadly applicable to biosynthetic pathways, including simultaneous control of different genes. It is also a powerful tool for balancing growth and production. We had previously engineered a modular autoinduction device for the control of gene expression in B. subtilis. Now, we expand its functionality to repress gene expression autonomously. The engineered R8 promoter responds to AHL accumulation in the culture medium. In a riboflavin-producing strain, the AHL-Lux complex exerts 5-fold repression on the R8-driven expression of the flavokinase/FAD synthetase gene ribC, resulting in a higher titer of the vitamin. We engineered a strain able to autonomously induce and repress different genes simultaneously, demonstrating the potential of the device for use in metabolic engineering.en
dc.description.affiliationDepartment of Bioprocess Engineering and Biotechnology School of Pharmaceutical Sciences Universidade Estadual Paulista (UNESP), Rodovia Araraquara-Jau km 1
dc.description.affiliationCenter for Natural and Human Sciences Federal University of ABC (UFABC), Campus Santo André, Avenida dos Estados 5001
dc.description.affiliationUnespDepartment of Bioprocess Engineering and Biotechnology School of Pharmaceutical Sciences Universidade Estadual Paulista (UNESP), Rodovia Araraquara-Jau km 1
dc.identifierhttp://dx.doi.org/10.3390/ijms24010084
dc.identifier.citationInternational Journal of Molecular Sciences, v. 24, n. 1, 2023.
dc.identifier.doi10.3390/ijms24010084
dc.identifier.issn1422-0067
dc.identifier.issn1661-6596
dc.identifier.scopus2-s2.0-85146023104
dc.identifier.urihttp://hdl.handle.net/11449/246629
dc.language.isoeng
dc.relation.ispartofInternational Journal of Molecular Sciences
dc.sourceScopus
dc.subjectautoinduction
dc.subjectautonomous control
dc.subjectBacillus subtilis
dc.subjectdynamic regulation
dc.subjectquorum-sensing
dc.subjectriboflavin
dc.titleExpanding the Functionality of an Autoinduction Device for Repression of Gene Expression in Bacillus subtilisen
dc.typeArtigo
unesp.author.orcid0000-0002-0005-096X[2]
unesp.author.orcid0000-0002-3034-6497[6]

Arquivos

Coleções