p-iodophenol-enhanced luminol chemiluminescent assay applied to discrimination between acute lymphoblastic and minimally differentiated acute myeloid (FAB-M0) or acute megakaryoblastic (FAB-M7) leukemias

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dc.contributor.authorda Costa, M.
dc.contributor.authorXimenes, Valdecir Farias [UNESP]
dc.contributor.authorBrunetti, Iguatemy Lourenço [UNESP]
dc.contributor.authorFalcao, R. P.
dc.contributor.authorFonseca, Luiz Marcos da [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-20T13:24:25Z
dc.date.available2014-05-20T13:24:25Z
dc.date.issued2004-01-01
dc.description.abstractIntroduction: In this report, we propose the application of the p-iodophenol-enhanced luminol chemiluminescent technique to the determination of peroxidase (myeloperoxidase and/or platelet peroxidase) activity in blasts of minimally differentiated acute myeloblastic leukemia (AML-M0) and acute megakaryoblastic leukemia (AML-M7).Methods: the frozen blast cells from 29 patients were thawed and submitted to the optimized protocol.Results: All cases of AML-M7 and AML-M0 exhibited integrated light emission greater than 73 (10(2) mV x s), which was the arbitrary cutoff point set for the discrimination between AML and acute lymphoblastic leukemia (ALL) (mean + 3 x s.d. of ALL samples, n = 10). In addition, five out of seven cases of AML-M0 showed results above the Cutoff point.Conclusion: This highly sensitive enhanced chemiluminescent technique may be applied to discriminate between ALL and AML-M7 or AML-M1 cases, and most AML-M0 cases. It is very simple, cheap and easy to perform compared to other procedures used to measure MPO activity in AML-leukemias including AML-M7 and AML-M0.en
dc.description.affiliationUNESP, Sch Pharmaceut Sci, Dept Clin Anal, Araraquara, SP, Brazil
dc.description.affiliationUniv São Paulo, Dept Clin Med, Sch Med, BR-14049 Ribeirao Preto, SP, Brazil
dc.description.affiliationUnespUNESP, Sch Pharmaceut Sci, Dept Clin Anal, Araraquara, SP, Brazil
dc.format.extent496-499
dc.identifierhttp://dx.doi.org/10.1038/sj.thj.6200562
dc.identifier.citationHematology Journal. London: Nature Publishing Group, v. 5, n. 6, p. 496-499, 2004.
dc.identifier.doi10.1038/sj.thj.6200562
dc.identifier.issn1466-4860
dc.identifier.lattes4419635633356792
dc.identifier.urihttp://hdl.handle.net/11449/7563
dc.identifier.wosWOS:000227379500007
dc.language.isoeng
dc.publisherNature Publishing Group
dc.relation.ispartofHematology Journal
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectacute myeloid leukemia (AML)pt
dc.subjectacute lymphoblastic leukemia (ALL)pt
dc.subjectchemiluminescencept
dc.subjectluminolpt
dc.subjectp-iodophenolpt
dc.subjectmyeloperoxidase (MPO)pt
dc.subjectplatelet peroxidase (PPO)pt
dc.titlep-iodophenol-enhanced luminol chemiluminescent assay applied to discrimination between acute lymphoblastic and minimally differentiated acute myeloid (FAB-M0) or acute megakaryoblastic (FAB-M7) leukemiasen
dc.typeArtigo
dcterms.licensehttp://www.nature.com/authors/policies/confidentiality.html
dcterms.rightsHolderNature Publishing Group
unesp.author.lattes4419635633356792
unesp.author.orcid0000-0002-2633-3909[4]
unesp.author.orcid0000-0003-4927-7599[3]
unesp.author.orcid0000-0003-2636-3080[2]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt

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