Muscle-specific growth hormone receptor (GHR) overexpression induces hyperplasia but not hypertrophy in transgenic zebrafish

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dc.contributor.authorFigueiredo, Marcio Azevedo
dc.contributor.authorMareco, Edson A. [UNESP]
dc.contributor.authorPai Silva, Maeli Dal [UNESP]
dc.contributor.authorMarins, Luis Fernando
dc.contributor.institutionUniv Fed Rio Grande
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:52:05Z
dc.date.available2014-05-20T13:52:05Z
dc.date.issued2012-06-01
dc.description.abstractEven though growth hormone (GH) transgenesis has demonstrated potential for improved growth of commercially important species, the hormone excess may result in undesired collateral effects. In this context, the aim of this work was to develop a new model of transgenic zebrafish () characterized by a muscle-specific overexpression of the GH receptor (GHR) gene, evaluating the effect of transgenesis on growth, muscle structure and expression of growth-related genes. In on line of transgenic zebrafish overexpressing GHR in skeletal muscle, no significant difference in total weight in comparison to non-transgenics was observed. This can be explained by a significant reduction in expression of somatotrophic axis-related genes, in special insulin-like growth factor I (IGF-I). In the same sense, a significant increase in expression of the suppressors of cytokine signaling 1 and 3 (SOCS) was encountered in transgenics. Surprisingly, expression of genes coding for the main myogenic regulatory factors (MRFs) was higher in transgenic than non-transgenic zebrafish. Genes coding for muscle proteins did not follow the MRFs profile, showing a significant decrease in their expression. These results were corroborated by the histological analysis, where a hyperplasic muscle growth was observed in transgenics. In conclusion, our results demonstrated that GHR overexpression does not induce hypertrophic muscle growth in transgenic zebrafish probably because of SOCS impairment of the GHR/IGF-I pathway, culminating in IGF-I and muscle proteins decrease. Therefore, it seems that hypertrophy and hyperplasia follow two different routes for entire muscle growth, both of them triggered by GHR activation, but regulated by different mechanisms.en
dc.description.affiliationUniv Fed Rio Grande, Inst Ciencias Biol, BR-96201900 Rio Grande, RS, Brazil
dc.description.affiliationUniv Fed Rio Grande, Inst Oceanog, Programa Posgrad Aquicultura, BR-96201900 Rio Grande, RS, Brazil
dc.description.affiliationUniv Estadual Paulista, UNESP, Inst Biociencias, Dept Morfol, Botucatu, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, UNESP, Inst Biociencias, Dept Morfol, Botucatu, SP, Brazil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIdCNPq: 471437/2009-3
dc.format.extent457-469
dc.identifierhttp://dx.doi.org/10.1007/s11248-011-9546-2
dc.identifier.citationTransgenic Research. Dordrecht: Springer, v. 21, n. 3, p. 457-469, 2012.
dc.identifier.doi10.1007/s11248-011-9546-2
dc.identifier.issn0962-8819
dc.identifier.urihttp://hdl.handle.net/11449/18611
dc.identifier.wosWOS:000304097400001
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofTransgenic Research
dc.relation.ispartofjcr2.197
dc.relation.ispartofsjr0,904
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectTransgenic zebrafishen
dc.subjectGrowth hormone receptoren
dc.subjectInsulin-like growth factor Ien
dc.subjectSuppressors of cytokine signalingen
dc.subjectHyperplasiaen
dc.subjectSkeletal muscleen
dc.titleMuscle-specific growth hormone receptor (GHR) overexpression induces hyperplasia but not hypertrophy in transgenic zebrafishen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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