Validação de método de análise quantitativa para a triancinolona a partir de complexo ternário por espectrofotometria de UV-Vis

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dc.contributor.authorAquino, G. D A
dc.contributor.authorStopilha, R. T.
dc.contributor.authorPedrosa, M. F F
dc.contributor.authorSantos, K. S C R
dc.contributor.authorEgito, E. S T
dc.contributor.authorOliveira, Anselmo Gomes de [UNESP]
dc.contributor.authorSilva-Junior, A. A.
dc.contributor.institutionTecBioFar
dc.contributor.institutionUniversidade Federal do Rio Grande do Norte (UFRN)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:25:55Z
dc.date.available2014-05-27T11:25:55Z
dc.date.issued2011-06-28
dc.description.abstractTriamcinolone (TRI), a drug widely used in the treatment of ocular inflammatory diseases, is practically insoluble in water, which limits its use in eye drops. Cyclodextrins (CDs) have been used to increase the solubility or dissolution rate of drugs. The purpose of the present study was to validate a UV-Vis spectrophotometric method for quantitative analysis of TRI in inclusion complexes with beta-cyclodextrin (B-CD) associated with triethanolamine (TEA) (ternary complex). The proposed analytical method was validated with respect to the parameters established by the Brazilian regulatory National Agency of Sanitary Monitoring (ANVISA). The analytical measurements of absorbance were made at 242nm, at room temperature, in a 1-cm path-length cuvette. The precision and accuracy studies were performed at five concentration levels (4, 8, 12, 18 and 20μg.mL -1). The B-CD associated with TEA did not provoke any alteration in the photochemical behavior of TRI. The results for the measured analytical parameters showed the success of the method. The standard curve was linear (r2 > 0.999) in the concentration range from 2 to 24 μg.mL -1. The method achieved good precision levels in the inter-day (relative standard deviation-RSD <3.4%) and reproducibility (RSD <3.8%) tests. The accuracy was about 80% and the pH changes introduced in the robustness study did not reveal any relevant interference at any of the studied concentrations. The experimental results demonstrate a simple, rapid and affordable UV-Vis spectrophotometric method that could be applied to the quantitation of TRI in this ternary complex.en
dc.description.affiliationLaboratório de Tecnologia e Biotecnologia Farmacêutica TecBioFar Departamento de Farmácia UFRN, Av. Cel. Gustavo Cordeiro de Farias, SN, Natal, RN, CEP 59180-010
dc.description.affiliationCiências Farmacêuticas UFRN, Natal, RN
dc.description.affiliationCiências Farmacêuticas Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista-Unesp, Araraquara, SP
dc.description.affiliationUnespCiências Farmacêuticas Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista-Unesp, Araraquara, SP
dc.format.extent35-40
dc.identifierhttp://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/view/1366
dc.identifier.citationRevista de Ciencias Farmaceuticas Basica e Aplicada, v. 32, n. 1, p. 35-40, 2011.
dc.identifier.file2-s2.0-79959532437.pdf
dc.identifier.issn1808-4532
dc.identifier.lattes9114495952533044
dc.identifier.scopus2-s2.0-79959532437
dc.identifier.urihttp://hdl.handle.net/11449/72500
dc.language.isoeng
dc.language.isopor
dc.relation.ispartofRevista de Ciências Farmacêuticas Básica e Aplicada
dc.relation.ispartofsjr0,131
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectBeta-cyclodextrin
dc.subjectTernary complexes
dc.subjectTriamcinolone
dc.subjectUV- Vis spectrophotometry
dc.subjectValidation
dc.subjectbeta cyclodextrin
dc.subjectternary complex factor
dc.subjecttriamcinolone
dc.subjecttriethanolamine
dc.subjectaccuracy
dc.subjectanalytic method
dc.subjectpH
dc.subjectphotochemistry
dc.subjectquantitative analysis
dc.subjectroom temperature
dc.subjectultraviolet spectrophotometry
dc.subjectvalidation study
dc.titleValidação de método de análise quantitativa para a triancinolona a partir de complexo ternário por espectrofotometria de UV-Vispt
dc.title.alternativeValidation of quantitative analysis method for triamcinolone in ternary complexes by UV-Vis spectrophotometryen
dc.typeArtigo
dcterms.licensehttp://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/about
unesp.author.lattes9114495952533044[6]
unesp.author.orcid0000-0002-0107-9940[6]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt
unesp.departmentFármacos e Medicamentos - FCFpt

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