Activity of silver nanoparticles on prokaryotic cells and Bothrops jararacussu snake venom

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dc.contributor.authorOliveira, Isadora Caruso Fontana
dc.contributor.authorde Paula, Marina Oliveira
dc.contributor.authorLastra, Hellen Cristine Boschilha
dc.contributor.authorAlves, Bruno de Brito
dc.contributor.authorMoreno, Débora Antunes Neto
dc.contributor.authorYoshida, Edson Hideaki
dc.contributor.authorAmaral Filho, Jorge
dc.contributor.authorCogo, José Carlos
dc.contributor.authorVaranda, Eliana Aparecida
dc.contributor.authorRai, Mahendra
dc.contributor.authorSantos, Carolina Alves dos
dc.contributor.authorOshima-Franco, Yoko
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T16:54:19Z
dc.date.available2018-12-11T16:54:19Z
dc.date.issued2018-06-29
dc.description.abstractNanoparticle-conjugated venom-toxins of venomous animals and its therapeutic efficacy against emerging or neglecting diseases is a promising strategy. In this study, silver nanoparticles (AgNPs ∼50 nm, 0.081 mg mL−1) were studied against the neuromuscular blockade, myotoxic effects induced by Bothrops jararacussu venom (60 µg mL−1) and also against prokaryotic cells. The neurotoxicity was evaluated on ex vivo mouse phrenic nerve-diaphragm using traditional myographic technique, able to obtain functional contractile responses and to check the neurotransmission. The myotoxicity on mammalian cells was evaluated in muscles resulting from pharmacological assays using routine histological techniques and light microscopy. The toxicity to prokaryotic cells was evaluated on Salmonella typhimurium TA100 without metabolic activation. The in vitro preincubation model between AgNPs and venom was enough to abolish toxic effects of B. jararacussu venom, but mammalian cells were highly sensitive to AgNPs more than prokaryotic cells, by acting as dose-independently and dose-dependently parameters, respectively. These results allowed us to conclude that AgNPs showed promising activity as antivenom agent but for its safer use, the toxicity should be evaluated on experimental animals.en
dc.description.affiliationVeterinary Medicine Graduate Course, University of Sorocaba (UNISO), Sorocaba, Brazil
dc.description.affiliationBioprocess and Biotechnology Engineering Course, University of Sorocaba (UNISO), Sorocaba, Brazil
dc.description.affiliationPost-Graduate Program in Pharmaceutical Sciences, University of Sorocaba (UNISO), Sorocaba, Brazil
dc.description.affiliationBioengineering and Biomedical Engineering Programs, Technological and Scientific Institute, Brazil University, Itaquera, São Paulo, Brazil
dc.description.affiliationPharmaceutical Sciences Faculty of Araraquara, São Paulo State University (UNESP), Araraquara, Brazil
dc.description.affiliationDepartment of Biotechnology, SGB Amravati University, Amravati, India
dc.format.extent1-5
dc.identifierhttp://dx.doi.org/10.1080/01480545.2018.1478850
dc.identifier.citationDrug and Chemical Toxicology, p. 1-5.
dc.identifier.doi10.1080/01480545.2018.1478850
dc.identifier.issn1525-6014
dc.identifier.issn0148-0545
dc.identifier.scopus2-s2.0-85049605363
dc.identifier.urihttp://hdl.handle.net/11449/171193
dc.language.isoeng
dc.relation.ispartofDrug and Chemical Toxicology
dc.relation.ispartofsjr0,460
dc.relation.ispartofsjr0,460
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectBothrops jararacussu venom
dc.subjectmyotoxicity
dc.subjectneurotoxicity
dc.subjectSalmonella typhimurium
dc.subjectsilver nanoparticles
dc.titleActivity of silver nanoparticles on prokaryotic cells and Bothrops jararacussu snake venomen
dc.typeArtigo
unesp.author.orcid0000-0002-4312-5220[11]

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