Analysis of experimental tendinitis in rats treated with laser and platelet-rich plasma therapies by Raman spectroscopy and histometry

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dc.contributor.authorCarvalho, Paula Kariluce de
dc.contributor.authorSilveira, Landulfo
dc.contributor.authorBarbosa, Danillo
dc.contributor.authorMunin, Egberto
dc.contributor.authorSalgado, Miguel Angel Castillo [UNESP]
dc.contributor.authorVillaverde, Antonio Balbin
dc.contributor.institutionUniversidade Camilo Castelo Branco (UNICASTELO)
dc.contributor.institutionCentro Universitário de Itajubá (FEPI)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2015-12-07T15:39:48Z
dc.date.available2015-12-07T15:39:48Z
dc.date.issued2015-10-24
dc.description.abstractThe objective of this controlled experimental study was to analyze the changes in the Achilles tendons of rats with experimentally induced tendinitis after treatment with platelet-rich plasma (PRP) and/or laser therapies by histometry to quantify fibroblasts and by Raman spectroscopy to determine the biochemical concentration of collagen types I and III. Fifty-four male Wistar rats were divided into six treatment groups: control (G1); PRP only (G2); irradiation with 660 nm laser (G3); irradiation with 830 nm laser (G4); PRP plus 660 nm laser irradiation (G5); and PRP plus 830 nm laser irradiation (G6). Injuries (partial tenotomy) were inflicted in the middle third of the Achilles tendon, with PRP added prior to suture in the appropriate experimental groups. A diode laser (model Laser Flash® III, DMC Equipamentos Ltda, São Carlos, SP, Brazil) that can be operated in two wavelengths 660 and 830 nm was used for irradiation treatments. The irradiation protocol was energy density of 70 J/cm(2), 20 s irradiation time, and 0.028 cm(2) spot area, per point in three points in the injured. The histometry was made in micrographical images of the H&E stained sections and evaluated by ImageJ (version 1.46r)®. Raman spectra were collected using a dispersive spectrometer at 830 nm excitation, 200 mW power, and 10 s integration time (P-1 Raman system, Lambda Solutions, Inc. MA, USA). The relative amount of type I collagen was significantly greater in the PRP plus 830 nm laser irradiation group (468 ± 188) than in the control (147 ± 137), 630 nm laser only (191 ± 117), and 830 nm laser only (196 ± 106) groups (p < 0.01), while the quantity of type III collagen was significantly greater in the PRP-only group compared to both irradiated groups without PRP (p < 0.05). Treatment with PRP combined with irradiation at 830 nm resulted in a larger number of fibroblasts and increased concentration of type I collagen, thus accelerating the healing of the injured tendon.en
dc.description.affiliationInstituto de Engenharia Biomédica, Universidade Camilo Castelo Branco, UNICASTELO, Estrada Dr. Altino Bondesan, 500, Distrito de Eugênio de Melo, São José dos Campos, SP, 12247-016, Brazil.
dc.description.affiliationDepartamento de Fisioterapia, Centro Universitário de Itajubá, FEPI, Rua Dr. Antonio Braga Filho, 687, Itajubá, MG, 37501-002, Brazil.
dc.description.affiliationInstituto de Ciência e Tecnologia, Universidade Estadual Paulista, UNESP, Av. Eng. Francisco Jose Longo, 777, São José dos Campos, SP, 12245-000, Brazil.
dc.description.affiliationInstituto de Engenharia Biomédica, Universidade Camilo Castelo Branco, UNICASTELO, Estrada Dr. Altino Bondesan, 500, Distrito de Eugênio de Melo, São José dos Campos, SP, 12247-016, Brazil.
dc.description.affiliationUnespInstituto de Ciência e Tecnologia, Universidade Estadual Paulista, UNESP, Av. Eng. Francisco José Longo, 777, São José dos Campos, SP, 12245-000, Brazil.
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2009/018788-5
dc.format.extent1-8
dc.identifierhttp://dx.doi.org/10.1007/s10103-015-1819-1
dc.identifier.citationLasers In Medical Science, p. 1-8, 2015.
dc.identifier.doi10.1007/s10103-015-1819-1
dc.identifier.issn1435-604X
dc.identifier.lattes8235700830441386
dc.identifier.pubmed26498452
dc.identifier.urihttp://hdl.handle.net/11449/131663
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofLasers In Medical Science
dc.rights.accessRightsAcesso restrito
dc.sourcePubMed
dc.subjectAchilles tendonen
dc.subjectHistometryen
dc.subjectLaser therapyen
dc.subjectPlatelet-rich plasma (prp) therapyen
dc.subjectRaman spectroscopyen
dc.subjectTendinitisen
dc.titleAnalysis of experimental tendinitis in rats treated with laser and platelet-rich plasma therapies by Raman spectroscopy and histometryen
dc.typeArtigo
dcterms.rightsHolderSpringer
unesp.author.lattes8235700830441386
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Ciência e Tecnologia, São José dos Campospt

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