Publicação:
Evaluation of level of DNA damage in blood leukocytes of non-diabetic and diabetic rat exposed to cigarette smoke

dc.contributor.authorOrtiz Lima, Paula Helena
dc.contributor.authorSinzato, Yuri Karen
dc.contributor.authorSoares de Souza, Maricelma da Silva
dc.contributor.authorBraz, Mariana Gobbo [UNESP]
dc.contributor.authorRudge, Marilza Vieira Cunha [UNESP]
dc.contributor.authorDamasceno, Débora Cristina [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de Marília (UNIMAR)
dc.date.accessioned2014-05-20T13:35:04Z
dc.date.available2014-05-20T13:35:04Z
dc.date.issued2007-04-02
dc.description.abstractThe objective of the present study was to use the comet assay to evaluate the steady-state level of DNA damage in peripheral blood leukocytes from diabetic and non-diabetic female Wistar rats exposed to air or to cigarette smoke. A total of 20 rats were distributed into four experimental groups (n= 5 rats/group): non-diabetic (control) and diabetic exposed to filtered air; non-diabetic and diabetic exposed to cigarette smoke. A pancreatic beta (beta)-cytotoxic agent, streptozotocin (40 mg/kg b.w.) was used to induce experimental diabetes in rats. Rats placed into whole-body exposure chambers were exposed for 30 min to filtered air (control) or to tobacco smoke generated from 10 cigarettes, twice a day, for 2 months. At the end of the 2-month exposure period, each rat was anesthetized and humanely killed to obtain blood samples for genotoxicity analysis using the alkaline comet assay. Blood wleukocytes sampled from diabetic rats presented higher DNA damage values (tail moment =0.57 +/- 0.05; tail length =19.92 +/- 0.41, p < 0.05) compared to control rats (tail moment =0.34 +/- 0.02; tail length= 17.42 +/- 0.33). Non-diabetic (tail moment =0.43 +/- 0.04, p > 0.05) and diabetic rats (tail moment= 0.41 +/- 0.03, p > 0.05) exposed to cigarette smoke presented non-significant increases in DNA damage levels compared to control group. In conclusion, our data show that the exposure of diabetic rats to cigarette smoke produced no additional genotoxicity in peripheral blood cells of female Wistar rats. (c) 2007 Elsevier B.V. All rights reserved.en
dc.description.affiliationUNESP, Botucatu Sch Med, Dept Obstet & Gynecol, Lab Expt Res & Gynecol & Obstet, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUNESP, Botucatu Sch Med, Dept Pathol, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUniv Marilia, Marilia, SP, Brazil
dc.description.affiliationUnespUNESP, Botucatu Sch Med, Dept Obstet & Gynecol, Lab Expt Res & Gynecol & Obstet, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUnespUNESP, Botucatu Sch Med, Dept Pathol, BR-18618000 Botucatu, SP, Brazil
dc.format.extent117-122
dc.identifierhttp://dx.doi.org/10.1016/j.mrgentox.2006.12.004
dc.identifier.citationMutation Research-genetic Toxicology and Environmental Mutagenesis. Amsterdam: Elsevier B.V., v. 628, n. 2, p. 117-122, 2007.
dc.identifier.doi10.1016/j.mrgentox.2006.12.004
dc.identifier.issn1383-5718
dc.identifier.lattes6758680388835078
dc.identifier.lattes5530023010203804
dc.identifier.orcid0000-0003-4413-226X
dc.identifier.orcid0000-0002-9227-832
dc.identifier.urihttp://hdl.handle.net/11449/12030
dc.identifier.wosWOS:000245368200006
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofMutation Research: Genetic Toxicology and Environmental Mutagenesis
dc.relation.ispartofjcr1.996
dc.relation.ispartofsjr0,747
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectstreptozotocin (STZ)pt
dc.subjectdiabetespt
dc.subjectcomet assaypt
dc.subjectDNA damagept
dc.subjectcigarette smokept
dc.titleEvaluation of level of DNA damage in blood leukocytes of non-diabetic and diabetic rat exposed to cigarette smokeen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
dspace.entity.typePublication
unesp.author.lattes6758680388835078
unesp.author.lattes5530023010203804
unesp.author.orcid0000-0003-4413-226X[4]
unesp.author.orcid0000-0002-9227-832X[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt
unesp.departmentGinecologia e Obstetrícia - FMBpt
unesp.departmentPatologia - FMBpt

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