Impaired macrophages and failure of steroidogenesis and spermatogenesis in rat testes with cytokines deficiency induced by diacerein

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dc.contributor.authorde Oliveira, Salmo Azambuja
dc.contributor.authorCerri, Paulo Sérgio [UNESP]
dc.contributor.authorSasso-Cerri, Estela [UNESP]
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2022-05-01T09:00:56Z
dc.date.available2022-05-01T09:00:56Z
dc.date.issued2021-01-01
dc.description.abstractThe role of cytokines in testicular function under normal conditions has not been completely understood. Here, we evaluated testicular macrophages (TM), steroidogenesis by Leydig cells (LC) and seminiferous tubules integrity in cytokines-deficient rat testes induced by diacerein, an anti-inflammatory drug that inhibits interleukin-1 (IL-1) and tumor necrosis factor-alpha (TNF-α). Male rats received daily 100 mg/kg of diacerein (DIAG; n = 8) or saline (CG; n = 8) for 30 days. Serum testosterone (T) levels were measured and the seminiferous tubule (ST) area, epithelial area (EA), frequency of damaged ST and number of Sertoli cells (SC) were evaluated. TUNEL method and immunoreactions for detection of pro-IL-1β, TNF-α, steroidogenic acute regulatory protein (StAR), 17β-hydroxysteroid dehydrogenase (17β-HSD), androgen receptor (AR) and scavenger receptor for hemoglobin-haptoglobin complexes (CD163), a TM marker, were performed. Testicular AR, 17β-HSD and IL-1β levels were detected by Western blot. Data were submitted to Student t test (p ≤ 0.05). In DIAG, T and testicular AR, 17β-HSD and IL-1β levels decreased significantly (p < 0.05). The number of TUNEL-positive interstitial cells increased and LC showed weak StAR, 17β-HSD and AR immunoexpression in association with reduced IL-1β immunoexpression and number of CD163-positive TM in the interstitial tissue from diacerein-treated rats. Numerous damaged ST were found in DIAG, and reduction in the EA were associated with germ cells death. Moreover, the number of SC reduced and weak AR and TNF-α immunoexpression was observed in SC and germ cells, respectively. The cytokines deficiency induced by diacerein impairs TM, LC and spermatogenesis, and points to a role of IL-1β in steroidogenesis under normal conditions. In the ST, the weak AR and TNF-α immunoexpression in SC and germ cells, respectively, reinforces the idea that TNF-α plays a role in the SC androgenic control.en
dc.description.affiliationDepartment of Morphology and Genetics Federal University of São Paulo
dc.description.affiliationLaboratory of Histology and Embryology Department of Morphology Genetics Orthodontics and Pediatric Dentistry Dental School – São Paulo State University (UNESP), Rua Humaitá, 1680
dc.description.affiliationUnespLaboratory of Histology and Embryology Department of Morphology Genetics Orthodontics and Pediatric Dentistry Dental School – São Paulo State University (UNESP), Rua Humaitá, 1680
dc.identifierhttp://dx.doi.org/10.1007/s00418-021-02023-7
dc.identifier.citationHistochemistry and Cell Biology.
dc.identifier.doi10.1007/s00418-021-02023-7
dc.identifier.issn1432-119X
dc.identifier.issn0948-6143
dc.identifier.scopus2-s2.0-85114787048
dc.identifier.urihttp://hdl.handle.net/11449/233528
dc.language.isoeng
dc.relation.ispartofHistochemistry and Cell Biology
dc.sourceScopus
dc.subjectAnti-inflammatory
dc.subjectIL-1
dc.subjectLeydig cell
dc.subjectMacrophage
dc.subjectSpermatogenesis
dc.subjectTNF-α
dc.titleImpaired macrophages and failure of steroidogenesis and spermatogenesis in rat testes with cytokines deficiency induced by diacereinen
dc.typeArtigo
unesp.author.orcid0000-0003-3101-4635[3]

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