Molecular characterization of persistent subclinical mastitis-causing Staphylococcus aureus from dairy farms

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dc.contributor.authorGonçalves, Juliano Leonel
dc.contributor.authorLee, Sarah H. I.
dc.contributor.authorCamargo, Carlos H.
dc.contributor.authorZanella, Rosemeire Cobo
dc.contributor.authorSilva, Nathália C. C.
dc.contributor.authorRall, Vera. L. M. [UNESP]
dc.contributor.authorCue, Roger I.
dc.contributor.authordos Santos, Marcos V.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionMichigan State University
dc.contributor.institutionInstituto Adolfo Lutz (IAL)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionMcGill University
dc.date.accessioned2023-07-29T14:01:13Z
dc.date.available2023-07-29T14:01:13Z
dc.date.issued2023-06-01
dc.description.abstractThe study aimed to evaluate the genetic diversity of Staphylococcus aureus causing subclinical mastitis (SM) isolated from dairy cows and to assess the effect of the infection status (transient vs. persistent) on the milk and component yield. A total of six dairy farms in São Paulo state were used for the selection of cows with SM caused by S. aureus. S. aureus strains (n = 56) obtained from three biweekly aseptic mammary quarter milk samplings (n = 1140 from 95 cows) were subjected to MALDI-TOF MS analysis for species confirmation and further PFGE analysis. Intramammary infections (IMI) caused by S. aureus were categorized as transient (T: when only one out of 3 milk samplings had positive isolation of any pulsotype) or persistent (P: when two (P2) or three (P3) milk samplings had positive isolation of identical pulsotype over the consecutive episodes of SM. The SmaI macrorestriction fragment profiles of 56 S. aureus isolates showed a dominant S. aureus clonal pattern (PFGE type A; n = 50; 89.3%) within and among the herds. The SM-causing S. aureus represented a reduction of quarter milk yield of 26.2% in transient and 54.8% in persistent cases as well as a reduction of total solid yield of 38.1% and 49.4%, respectively, when compared with the healthy control quarters. Overall, the greater chance of S. aureus to be persistent is when a dominant clonal pattern is present in the herd which consequently may be associated with the cause of accentuated milk loss.en
dc.description.affiliationDepartment of Animal Nutrition and Production School of Veterinary Medicine and Animal Sciences University of Sao Paulo, São Paulo
dc.description.affiliationDepartment of Large Animal Clinical Sciences College of Veterinary Medicine Michigan State University
dc.description.affiliationDepartment of Food Engineering Faculty of Animal Science and Food Engineering University of São Paulo, São Paulo
dc.description.affiliationCentro de Bacteriologia Instituto Adolfo Lutz (IAL), SP
dc.description.affiliationDepartment of Food Science and Nutrition Faculty of Food Engineering University of Campinas, São Paulo
dc.description.affiliationDepartment of Chemical and Biological Sciences Institute of Biosciences Sao Paulo State University, São Paulo
dc.description.affiliationDepartment of Animal Science McGill University, Macdonald Campus
dc.description.affiliationUnespDepartment of Chemical and Biological Sciences Institute of Biosciences Sao Paulo State University, São Paulo
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: BEPE 2015/04570-1)
dc.description.sponsorshipIdFAPESP: project funding (2014/17411-6)
dc.description.sponsorshipIdFAPESP: The authors ackledge FAPESP for the scholarship (2013/23613-8
dc.format.extent1181-1189
dc.identifierhttp://dx.doi.org/10.1007/s42770-023-00918-2
dc.identifier.citationBrazilian Journal of Microbiology, v. 54, n. 2, p. 1181-1189, 2023.
dc.identifier.doi10.1007/s42770-023-00918-2
dc.identifier.issn1678-4405
dc.identifier.issn1517-8382
dc.identifier.scopus2-s2.0-85150509918
dc.identifier.urihttp://hdl.handle.net/11449/249055
dc.language.isoeng
dc.relation.ispartofBrazilian Journal of Microbiology
dc.sourceScopus
dc.subjectChronic infection
dc.subjectGenetic diversity
dc.subjectMilk reduction
dc.subjectPFGE
dc.subjectStaphylococci
dc.titleMolecular characterization of persistent subclinical mastitis-causing Staphylococcus aureus from dairy farmsen
dc.typeArtigo
unesp.author.orcid0000-0003-2138-4982[1]

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Artigos - Melhoramento e Nutrição Animal - FMVZ