Departamento: Departamento de Ciências Biológicas
Carregando...
Sigla
DCB
Unidades
Faculdade de Ciências Farmacêuticas
FCF
Campus: Araraquara
546 resultados
Resultados da pesquisa de publicação
Agora exibindo 1 - 10 de 546
PublicaçãoArtigo Triatominae survey (Hemiptera: Reduviidae: Triatominae) in the south-central region of the state of Bahia, Brazil between 2008 and 2013(Amer Soc Trop Med &hygiene, 2015-05-01) Mendonca, Vagner José ; Oliveira, Jader de
; Rimoldi, Aline ; Ferreira Filho, Julio César Rente ; Araujo, Renato Freitas de ; Rosa, João Aristeu da ; Universidade de Brasília (UnB) ; Universidade Estadual Paulista (Unesp) ; Universidade Estadual de Campinas (UNICAMP) ; Universidade de São Paulo (USP) ; Secretaria Estadual de Saúde da Bahia (SESAB) Triatomine surveillance in rural areas, artificial ecotypes, and natural ecotopes of the cities of Caturama, Ibipitanga, Macaubas, and Seabra in the south-central region of the Brazilian state of Bahia was carried out between 2008 and 2013. Natural infection by Trypanosoma cruzi was evaluated in the specimens collected to monitor vectors of Chagas disease. A total of 1,357 specimens were collected, and four species were identified: Triatoma sordida (83%), Triatoma lend (16.4%), Triatoma pseudomaculata (0.5%), and Panstrongylus geniculatus (0.1%). Triatoma sordida was found in four cities, only 0.7% in intradomiciliary environments. Triatoma lend was found only in Macaubas; 8.5% were found in intradomiciliary environments, 88.3% in peridomiciliary environments, and 3.1% in sylvatic environments. Natural infection by T. cruzi was 0.5% for T. sordida and 3.1% T. lenti. All of these cases were found in peridomiciliary environments of Macaubas. As the results show, triatomines were found in intradomiciliary environments in three cities that were surveyed in the south-central region of the state of Bahia. Thus, an epidemiologic survey should be performed to avoid the risk of transmission to the population.PublicaçãoArtigo 
Acesso Aberto Leukotrienes are not essential for the efficacy of a heterologous vaccine against Mycobacterium tuberculosis infection(Associação Brasileira de Divulgação Científica (ABRADIC), 2010-07-01) Franco, L. H. ; Paula, M. Oliveira e ; Wowk, P. F. ; Fonseca, D. M. da ; Sérgio, C. A. ; Fedatto, P. F. ; Gembre, A. F. ; Ramos, S. G. ; Silva, C. L. ; Medeiros, Alexandra Ivo de; Faccioli, L. H. ; Bonato, V. L. D. ; Universidade de São Paulo (USP) ; Universidade Estadual Paulista (Unesp) Leukotrienes are reported to be potent proinflammatory mediators that play a role in the development of several inflammatory diseases such as asthma, rheumatoid arthritis and periodontal disease. Leukotrienes have also been associated with protection against infectious diseases. However, the role of leukotrienes in Mycobacterium tuberculosis infection is not understood. To answer this question, we studied the role of leukotrienes in the protective immune response conferred by prime-boost heterologous immunization against tuberculosis. We immunized BALB/c mice (4-11/group) with subcutaneous BCG vaccine (1 x 10(5) M. bovis BCG) (prime) followed by intramuscular DNA-HSP65 vaccine (100 µg) (boost). During the 30 days following the challenge, the animals were treated by gavage daily with MK-886 (5 mg·kg-1·day-1) to inhibit leukotriene synthesis. We showed that MK-886-treated mice were more susceptible to M. tuberculosis infection by counting the number of M. tuberculosis colony-forming units in lungs. The histopathological analysis showed an impaired influx of leukocytes to the lungs of MK-886-treated mice after infection, confirming the involvement of leukotrienes in the protective immune response against experimental tuberculosis. However, prime-boost-immunized mice treated with MK-886 remained protected after challenge with M. tuberculosis, suggesting that leukotrienes are not required for the protective effect elicited by immunization. Protection against M. tuberculosis challenge achieved by prime-boost immunization in the absence of leukotrienes was accompanied by an increase in IL-17 production in the lungs of these animals, as measured by ELISA. Therefore, these data suggest that the production of IL-17 in MK-886-treated, immunized mice could contribute to the generation of a protective immune response after infection with M. tuberculosis.PublicaçãoArtigo Antimycobacterial activity of Indigofera suffruticosa with activation potential of the innate immune system(Taylor & Francis Inc, 2010-08-01) Carli, Camila B. de A.; Quilles, Marcela B. ; Maia, Daniele C. G. ; Lopes, Flavia C. M. ; Santos, Rubens ; Pavan, Fernando R. ; Fujimura Leite, Clarice Q. ; Calvo, Tamara Regina ; Vilegas, Wagner ; Carlos, Iracilda Z. ; Universidade Estadual Paulista (Unesp) Mycobacterium tuberculosis is responsible for over 8 million cases of tuberculosis (TB) annually. Natural products may play important roles in the chemotherapy of TB. The antimycobacterial activity and the innate immune response of methanol (METH) and dichloromethane (DCM) extracts of Indigofera suffruticosa Miller (Fabaceae) were evaluated. We observed that the minimum inhibitory concentrations (MICs) for METH and DCM extracts were 125 and 1000 mu g/mL, respectively. However, they were able to induce the innate immune response through the production of high levels of NO and TNF-alpha (p < 0.001) by peritoneal exudate cells (PECs). These results suggest that I. suffruticosa extracts may have an important immunological role in the control of TB once macrophage activity is induced by them.PublicaçãoArtigo Acesso Aberto Acesso, segurança e uso de medicamentos por usuários(2012) Andrade, Raul Vasconcelos; Farache Filho, Adalberto ; Galduróz, José Carlos ; Mastroianni, Patricia de Carvalho ; Universidade Estadual Paulista (Unesp) ; Universidade Federal de São Paulo (UNIFESP) The present study aims to identify families who have stock and are making use of medications, as well as assess the conditions of storage, security and use of these drugs. The study was conducted in a city of São Paulo, the interviews were conducted in households enrolled in one of ten units of the Estratégia de Saúde da Família (ESF) that the city provides and the sample was defined by means of stratified random sampling (134households, IC 95%). Data collection was conducted through interviews with a semistructured questionnaire during the first half of 2011. We interviewed 118 (88.0%) households, of which 112 (95.0%) had medications that were stored n insecure or inadequate places in 75.4% of households, non-prescription self-medication was a common practice in 46 (47 4%) households, and lack of identification and security of medications stored was observed in 60 (53.6%) households. Most households had stock of medicines, which were done improperly or unsecure, or have specialties with lack of identification and security, which can lead to poisoning or e ineffective therapy. The Pharmaceutical Assistance under SUS lacks social initiatives, with actions directed for medications users, which can be supplied by the presence of the pharmacist in the ESF, essential for the promotion of racional use of medicines, that, through the Pharmaceutical Care, can identify, correct and prevent possible problems related to drugs.PublicaçãoArtigo Acesso Aberto Using captive sentinels to collect wild triatomines in the region of Marília-SP, Brasil(2013) Martins, Luciamáre Perrinetti Alves ; Castanho, Roberto Esteves Pires ; Tonon, Felipe Frasão ; Unterleider, Carlos Eduardo ; Rosa, João Aristeu da; Universidade Estadual Paulista (Unesp) In order to collect wild triatomines in the municipality of Marilia, two captive sentinel sites were installed in a remnant riparian zone in the Atlantic Forest comprising 34 hectares located on the Nossa Senhora Aparecida farm. One enclosure was built on the margin and another inside the forest, about 200 meters from the edge, using rabbits as bait to attract wild triatomines. In addition to the caged rabbits, active searches for triatomines were performed in tree holes, bird nests and armadillo burrows by spraying Pirisa 1080-L1E1 aerosol dislodger. The sentinels were maintained for one year and four months. During this period six active searches were performed, but without success in attracting or colonizing wild triatomines. One factor that has probably intervened in the triatomine colonization is the periodic application of insecticides formulated with pyrethroid in farms neighboring the forest to fight Diaphorina citri in orange plantations.PublicaçãoArtigo Acesso Aberto Soropositividade para doença de Chagas entre doadores de sangue em Araraquara, Estado de São Paulo, no período de 2004 a 2008(Sociedade Brasileira de Medicina Tropical - SBMT, 2011-02-01) Ferreira Filho, Julio César Rente; Costa, Paulo Inácio da ; Buainain, Arnaldo ; Rosa, João Aristeu da ; Universidade Estadual Paulista (Unesp) ; Laboratório Dr. Arnaldo Buainain INTRODUÇÃO: A transfusão sanguínea é uma fonte potencial importante para transmissão da doença de Chagas. MÉTODOS: Foi verificado, nos arquivos do Hemonúcleo Regional de Araraquara, o resultado dos exames para doença de Chagas entre janeiro de 2004 e dezembro de 2008. RESULTADOS: Foram diagnosticadas com sorologia positiva 0,04% das 49541 doações de sangue realizadas. A idade dos soropositivos situou-se entre 51 e 60 anos. CONCLUSÕES: O baixo índice de doadores soropositivos pode reduzir o risco de transmissão via transfusional da doença de Chagas. A alta ocorrência de resultados inconclusivos indicam que os métodos diagnósticos devem ser melhorados.PublicaçãoArtigo Acesso Aberto Impairment of neutrophil migration to remote inflammatory site during lung histoplasmosis(Hindawi Publishing Corporation, 2015-01-01) Medeiros, Alexandra Ivo de; Secatto, Adriana ; Belanger, Caroline ; Sorgi, Carlos Artério ; Borgeat, Pierre ; Marleau, Sylvie ; Faccioli, Lucia Helena ; Universidade Estadual Paulista (Unesp) ; Universidade de São Paulo (USP) ; Université de Montréal ; Centre Hospitalier Universitaire de Québec (CHUQ) Histoplasma capsulatum (Hc) induces a pulmonary disease in which leukotrienes promote activation and recruitment of effectors cells. It is also well-recognized that leukotriene B 4 (LTB 4) and platelet-activating factor (PAF) induce leukocyte recruitment to inflammatory sites. We investigated the impact of pulmonary Hc infection on PMN migration to a remote inflammatory site. Our results show that pulmonary Hc infection impairs LTB4- or PAF-stimulated PMN recruitment to air pouch. Yet, remote inflammation did not modify PMN numbers in the bronchoalveolar lavage fluid (BALF) of Hc-infected mice. Interestingly, the concomitant administration of PAF and LTB 4 receptor antagonists inhibited PMN recruitment to both BALF and the remote site, demonstrating cooperation between both mediators. Along that line, our results show that PAF-elicited PMN chemotaxis was abrogated in 5-lipoxygenase-deficient animals. These results suggest caution in the indiscriminate use of anti-inflammatory drugs during infectious diseases.PublicaçãoArtigo eIF5A and EF-P: two unique translation factors are now traveling the same road(Wiley-Blackwell, 2014-03-01) Rossi, Danuza; Kuroshu, Reginaldo ; Zanelli, Cleslei Fernando ; Valentini, Sandro Roberto ; Universidade Estadual Paulista (Unesp) ; Universidade Federal de São Paulo (UNIFESP) Translational control is extremely important in all organisms, and some of its aspects are highly conserved among all primary kingdoms, such as those related to the translation elongation step. The previously classified translation initiation factor 5A (eIF5A) and its bacterial homologue elongation factor P (EF-P) were discovered in the late 70's and have recently been the object of many studies. eIF5A and EF-P are the only cellular proteins that undergo hypusination and lysinylation, respectively, both of which are unique posttranslational modifications. Herein, we review all the important discoveries related to the biochemical and functional characterization of these factors, highlighting the implication of eIF5A in translation elongation instead of initiation. The findings that eIF5A and EF-P are important for specific cellular processes and play a role in the relief of ribosome stalling caused by specific amino acid sequences, such as those containing prolines reinforce the hypothesis that these factors are involved in specialized translation. Although there are some divergences between these unique factors, recent studies have clarified that they act similarly during protein synthesis. Further studies may reveal their precise mechanism of ribosome activity modulation as well as the mRNA targets that require eIF5A and EF-P for their proper translation. (C) 2014 John Wiley & Sons, Ltd.PublicaçãoArtigo Acesso Aberto The Leukotriene B-4/BLT1 Axis Is a Key Determinant in Susceptibility and Resistance to Histoplasmosis(Public Library Science, 2014-01-21) Secatto, Adriana ; Soares, Elyara Maria ; Locachevic, Gisele Aparecida ; Assis, Patricia Aparecida ; Garcia Paula-Silva, Francisco Wanderlei ; Serezani, Carlos Henrique ; Medeiros, Alexandra Ivo de; Faccioli, Lucia Helena ; Universidade de São Paulo (USP) ; Indiana University ; Universidade Estadual Paulista (Unesp) The bioactive lipid mediator leukotriene B-4 (LTB4) greatly enhances phagocyte antimicrobial functions against a myriad of pathogens. In murine histoplasmosis, inhibition of the LT-generating enzyme 5-lypoxigenase (5-LO) increases the susceptibility of the host to infection. In this study, we investigated whether murine resistance or susceptibility to Histoplasma capsulatum infection is associated with leukotriene production and an enhancement of in vivo and/or in vitro antimicrobial effector function. We show that susceptible C57BL/6 mice exhibit a higher fungal burden in the lung and spleen, increased mortality, lower expression levels of 5-LO and leukotriene B-4 receptor 1 (BLT1) and decreased LTB4 production compared to the resistant 129/Sv mice. Moreover, we demonstrate that endogenous and exogenous LTs are required for the optimal phagocytosis of H. capsulatum by macrophages from both murine strains, although C57BL/6 macrophages are more sensitive to the effects of LTB4 than 129/Sv macrophages. Therefore, our results provide novel evidence that LTB4 production and BLT1 signaling are required for a histoplasmosis-resistant phenotype.PublicaçãoArtigo Acesso Aberto Comparison of resazurin microtiter assay performance and BACTEC MGIT 960 in the susceptibility testing of Brazilian clinical isolates of Mycobacterium tuberculosis to four first-line drugs(Sociedade Brasileira de Microbiologia, 2013-01-01) Miyata, Marcelo; Pavan, Fernando Rogério ; Sato, Daisy Nakamura ; Marino, Leonardo Biancolino ; Hirata, Mario Hiroyuki ; Cardoso, Rosilene Fressati ; Melo, Fernando Augusto Fiúza de ; Leite, Clarice Queico Fujimura ; Universidade Estadual Paulista (Unesp) ; Universidade de São Paulo (USP) ; Universidade Estadual de Maringá (UEM) ; Instituto Clemente Ferreira We assessed the performance of REMA in comparison with BACTEC MGIT 960 in the susceptibility testing of 80 Mycobacterium tuberculosis clinical isolates from Clemente Ferreira Institute against four drugs. REMA proved to be a rapid and accurate method, providing excellent correlation with BACTEC MGIT 960, with the exception of results for the ethambutol drug.