GLUCOCORTICOID-REGULATED GENE IN TRANSFORMED TO NORMAL PHENOTYPIC REVERSION
Loading...
Files
Date
Advisor
Coadvisor
Graduate program
Undergraduate course
Journal Title
Journal ISSN
Volume Title
Publisher
Associação Brasileira de Divulgação Científica (ABRADIC)
Type
Article
Access right
Acesso restrito
Files
Abstract
Glucocorticoid hormones modulate the actions of peptide growth factors and constitute important therapeutic tools as anti-inflammatory and anti-tumor agents. The C6 rat glioma cell line responds to glucocorticoids with changes in morphology and growth block. The hyper-responsive ST1 cell variant displays a dramatic phenotypic reversion under the influence of these hormones. Thus, the transformed and tumorigenic cells reversibly change to a normal and non-tumorigenic phenotype. In addition, the cells also produce a C-type retrovirus. We used poly A(+) mRNA from ST1 cells that had been treated with hydrocortisone to generate a cDNA library that was then screened, by differential hybridization,for glucocorticoid-responsive cellular sequences. The retroviral genomic RNA was used to generate a viral-specific probe. Cross hybridization led to the isolation of at least 4 cDNA clones of which 3 are cellular sequences and one corresponds to a retroviral gene. These clones were characterized by DNA sequencing and Northern blot hybridization analysis.
Description
Keywords
CDNA CLONING, DIFFERENTIAL HYBRIDIZATION, GLUCOCORTICOID-REGULATED GENES
Language
English
Citation
Brazilian Journal of Medical and Biological Research. São Paulo: Associação Bras Divulg Cientifica, v. 27, n. 2, p. 541-546, 1994.
