Polymorphism of the ITS-2 region of the ribosomal DNA of the Triatominae Rhodnius domesticus, R-pictipes, R-prolixus and R-stali
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Blackwell Publishing
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The polymerase chain reaction-restriction fragment length polymorphism technique (PCR-RFLP) was used to compare Rhodnius domesticus (Neiva & Pinto), R. pictipes (Stal), R. prolixus (Stal) and R. stali (Lent; Jurberg & Galvao) (Hemiptera: Reduviidae). The enzyme BstUI differentiated R. donzesticus, R. pictipes and R. prolixus, and HhaI differentiated R. domesticus, R. pictipes and R. stali. With the fingerprinting analysis generated by these two enzymes, it was possible to clearly identify all four species in the study.
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Rhodnius, endonucleases, ITS-2, PCR-RFLP, Triatominae
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Inglês
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Medical and Veterinary Entomology. Oxford: Blackwell Publishing, v. 20, n. 4, p. 353-357, 2006.
