Anticardiolipin antibodies from syphilis and systemic lupus erythematosus induce leakage in cardiolipin vesicles
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Undergraduate course
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Associação Brasileira de Divulgação Científica (ABRADIC)
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Article
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Abstract
Anticardiolipin antibodies from sera of patients with systemic lupus erythematosus or syphilis induced leakage of entrapped carboxyfluorescein (CF) from cardiolipin (CL)/phosphatidylcholine(PC) vesicles prepared by sonication of equimolar mixtures of CL:PC. The sera dilution used here was 1:7500. IgG (5-20 mu g/ml) from the same sera, not containing beta(2)GPI, also produced a concentration-dependent leak. Vesicle leakage was inhibited by salt and was not detected with vesicles prepared exclusively with phosphatidylcholine. The demonstration of antibody-induced vesicle leakage offers a convenient system to investigate the mechanism of antibody-lipid binding as well as a potential diagnostic tool.
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Keywords
anticardiolipin antibodies, cardiolipin, vesicles, syphilis, systemic lupus erythematosus, carboxyfluorescein
Language
English
Citation
Brazilian Journal of Medical and Biological Research. São Paulo: Associação Bras Divulg Cientifica, v. 29, n. 4, p. 489-494, 1996.
