Quantification of Doxycycline Hyclate in Tablets by HPLC-UV Method

Kogawa, Ana Carolina [UNESP]; Salgado, Hérida Regina Nunes [UNESP]

Quantification of Doxycycline Hyclate in Tablets by HPLC-UV Method

Data

2013-11-01

Autores

Kogawa, Ana Carolina

Salgado, Hérida Regina Nunes

Editor

Oxford University Press

Tipo

Artigo

Direito de acesso

Acesso restrito

Resumo

An accurate, sensitive, precise and rapid gradient reversed-phase high-performance liquid chromatographic method was successfully developed and validated for the determination of doxycycline hyclate in bulk drug and tablets. The best separation was achieved on a 250 x 4.6 mm, 5.0 mu m particle size CN Luna column with water + 0.1% TFA-acetonitrile + 0.1% TFA, 60:40 (v/v) as the mobile phase at a flow rate of 1.0 mL/min. Ultraviolet detection was performed at 360 nm at ambient column temperature (25 degrees C). The method was linear over the concentration range of 50-100 mg/mL (r = 50.9999) with limits of detection and quantification of 2.83 and 8.59 mu g/mL, respectively. The drug was subjected to oxidation, acid, base and neutral degradation, photolysis and heat as stress conditions. Degradation products were found interfering with the assay of doxycycline hyclate, therefore the method can be regarded as suitable. The method was applied for the determination of doxycycline hyclate in standard and pharmaceutical products, with excellent recoveries. The method can be used for the quality control of doxycycline hyclate.