Trypanosoma cruzi: establishment of permeable cells for RNA processing analysis with drugs
Loading...
External sources
External sources
Date
Advisor
Coadvisor
Graduate program
Undergraduate course
Journal Title
Journal ISSN
Volume Title
Publisher
Instituto Oswaldo Cruz, Ministério da Saúde
Type
Article
Access right
Acesso aberto
External sources
External sources
Abstract
Pre-mRNA maturation in trypanosomatids occurs through a process called trans-splicing which involves excision of introns and union of exons in two independent transcripts. For the first time, we present the standardization of Trypanosoma cruzi permeable cells (Y strain) as a model for trans-splicing study of mRNAs in trypanosomes, following by RNase protection reaction, which localizes the SL exon and intron. This trans-splicing reaction in vitro was also used to analyze the influence of NFOH-121, a nitrofurazone-derivative, on this mechanism. The results suggested that the prodrug affects the RNA processing in these parasites, but the trans-splicing reaction still occurred.
Description
Keywords
trans-splicing, Trypanosoma cruzi, NFOH-121 (hydroxymethylnitrofurazone)
Language
English
Citation
Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 99, n. 6, p. 617-620, 2004.
