Logotipo do repositório
 

Publicação:
Antimicrobial Properties and Cytotoxicity of an Antimicrobial Monomer for Application in Prosthodontics

dc.contributor.authorRegis, Romulo Rocha
dc.contributor.authorDella Vecchia, Maria Paula
dc.contributor.authorPizzolitto, Antonio Carlos [UNESP]
dc.contributor.authorCompagnoni, Marco Antonio [UNESP]
dc.contributor.authorChaves Souza, Pedro Paulo [UNESP]
dc.contributor.authorde Souza, Raphael Freitas
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:45:54Z
dc.date.available2014-05-20T13:45:54Z
dc.date.issued2012-06-01
dc.description.abstractPurpose: This study aimed to investigate the antimicrobial properties and cytotoxicity of the monomer methacryloyloxyundecylpyridinium bromide (MUPB), an antiseptic agent capable of copolymerizing with denture base acrylic resins. Materials and Methods: The antimicrobial activity of MUPB was tested against the species Candida albicans, Candida dubliniensis, Candida glabrata, Lactobacillus casei, Staphylococcus aureus, and Streptococcus mutans. The minimum inhibitory and fungicidal/bactericidal concentrations (MIC, MFC/MBC) of MUPB were determined by serial dilutions in comparison with cetylpyridinium chloride (CPC). The cytotoxic effects of MUPB at concentrations ranging from 0.01 to 1 g/L were assessed by MTT test on L929 cells and compared with methyl methacrylate (MMA). The antimicrobial activity of copolymerized MUPB was tested by means of acrylic resin specimens containing three concentrations of the monomer (0, 0.3, 0.6% w/w). Activity was quantified by means of a disc diffusion test and a quantification of adhered planktonic cells. Statistical analysis employed the Mann-Whitney test for MIC and MFC/MBC, and ANOVA for the microbial adherence test (a= 0.05). Results: MUBP presented lower MIC values when compared with CPC, although differences were significant for C. dubliniensis and S. mutans only (p= 0.046 and 0.043, respectively). MFC/MBC values were similar for all species except C. albicans; in that case, MUPB presented significantly higher values (p= 0.046). MUPB presented higher cytotoxicity than MMA for all tested concentrations (p < 0.001) except at 0.01 g/L. Irrespective of the concentration incorporated and species, there was no inhibition halo around the specimens. The incorporation of MUPB influenced the adhesion of C. albicans only (p= 0.003), with lower CFU counts for the 0.6% group. Conclusions: It was concluded that non-polymerized MUPB has an antimicrobial capacity close to that of CPC and high cytotoxicity when compared with MMA. The antimicrobial activity of MUPB after incorporation within a denture base acrylic resin did not depend on its elution, but was shown to be restricted to C. albicans.en
dc.description.affiliationUniv São Paulo, Dept Dent Mat & Prosthodont, Ribeirao Preto Dent Sch, BR-14049 Ribeirao Preto, Brazil
dc.description.affiliationSão Paulo State Univ, Sch Pharmaceut Sci Araraquara, Dept Clin Anal, Araraquara, Brazil
dc.description.affiliationSão Paulo State Univ, Dept Dent Mat & Prosthodont, Araraquara Dent Sch, Araraquara, Brazil
dc.description.affiliationSão Paulo State Univ, Dept Physiol & Pathol, Araraquara Dent Sch, Araraquara, Brazil
dc.description.affiliationUnespSão Paulo State Univ, Sch Pharmaceut Sci Araraquara, Dept Clin Anal, Araraquara, Brazil
dc.description.affiliationUnespSão Paulo State Univ, Dept Dent Mat & Prosthodont, Araraquara Dent Sch, Araraquara, Brazil
dc.description.affiliationUnespSão Paulo State Univ, Dept Physiol & Pathol, Araraquara Dent Sch, Araraquara, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 07/533477-7
dc.description.sponsorshipIdFAPESP: 07/05245-0
dc.format.extent283-290
dc.identifierhttp://dx.doi.org/10.1111/j.1532-849X.2011.00815.x
dc.identifier.citationJournal of Prosthodontics-implant Esthetic and Reconstructive Dentistry. Hoboken: Wiley-blackwell, v. 21, n. 4, p. 283-290, 2012.
dc.identifier.doi10.1111/j.1532-849X.2011.00815.x
dc.identifier.issn1059-941X
dc.identifier.lattes8921196002764597
dc.identifier.urihttp://hdl.handle.net/11449/16193
dc.identifier.wosWOS:000305510900006
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofJournal of Prosthodontics-implant Esthetic and Reconstructive Dentistry
dc.relation.ispartofjcr1.745
dc.rights.accessRightsAcesso restritopt
dc.sourceWeb of Science
dc.subjectLocal anti-infectious agentsen
dc.subjectdenture basesen
dc.subjectquaternary ammonium compoundsen
dc.subjectstomatitis under denturesen
dc.subjectacrylic resinsen
dc.titleAntimicrobial Properties and Cytotoxicity of an Antimicrobial Monomer for Application in Prosthodonticsen
dc.typeArtigopt
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderWiley-blackwell
dspace.entity.typePublication
relation.isDepartmentOfPublicationa83d26d6-5383-42e4-bb3c-2678a6ddc144
relation.isDepartmentOfPublication3936e2e2-946a-42ab-8b9d-9521513200fc
relation.isDepartmentOfPublicationb3ba3d9c-022e-4521-8805-0bcceea7372e
relation.isDepartmentOfPublication.latestForDiscoverya83d26d6-5383-42e4-bb3c-2678a6ddc144
relation.isOrgUnitOfPublication95697b0b-8977-4af6-88d5-c29c80b5ee92
relation.isOrgUnitOfPublicationca4c0298-cd82-48ee-a9c8-c97704bac2b0
relation.isOrgUnitOfPublication.latestForDiscovery95697b0b-8977-4af6-88d5-c29c80b5ee92
unesp.author.lattes8921196002764597
unesp.author.orcid0000-0001-7266-6061[5]
unesp.author.orcid0000-0001-8437-9991[6]
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Ciências Farmacêuticas, Araraquarapt
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Odontologia, Araraquarapt
unesp.departmentAnálises Clínicas - FCFpt
unesp.departmentFisiologia e Patologia - FOARpt
unesp.departmentMateriais Odontológicos e Prótese - FOARpt

Arquivos

Licença do Pacote

Agora exibindo 1 - 2 de 2
Carregando...
Imagem de Miniatura
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Carregando...
Imagem de Miniatura
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição: