Publicação: Anticardiolipin antibodies from syphilis and systemic lupus erythematosus induce leakage in cardiolipin vesicles
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Anticardiolipin antibodies from sera of patients with systemic lupus erythematosus or syphilis induced leakage of entrapped carboxyfluorescein (CF) from cardiolipin (CL)/phosphatidylcholine(PC) vesicles prepared by sonication of equimolar mixtures of CL:PC. The sera dilution used here was 1:7500. IgG (5-20 μg/ml) from the same sera, not containing B2GPI, also produced a concentration-dependent leak. Vesicle leakage was inhibited by salt and was not detected with vesicles prepared exclusively with phosphatidylcholine. The demonstration of antibody-induced vesicle leakage offers a convenient system to investigate the mechanism of antibody-lipid binding as well as a potential diagnostic tool.
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Anticardiolipin antibodies, Carboxyfluorescein, Cardiolipin, Syphilis, Systemic lupus erythematosus, Vesicles
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Inglês
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Brazilian Journal of Medical and Biological Research, v. 29, n. 4, p. 489-494, 1996.
