Publicação: LC Assay for Ciprofloxacin Hydrochloride Ophthalmic Solution
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Data
Orientador
Coorientador
Pós-graduação
Curso de graduação
Título da Revista
ISSN da Revista
Título de Volume
Editor
Vieweg
Tipo
Artigo
Direito de acesso
Acesso restrito
Resumo
The objective of the current study was to develop and subsequently validate a simple, sensitive and precise reversed-phase LC method for the determination of ciprofloxacin hydrochloride in ophthalmic solution form. The chromatographic separation of ciprofloxacin hydrochloride was achieved on a Symmetry Waters C(18) column using UV detection at 275 nm. The optimized mobile phase consisted of 2.5% acetic acid solution: methanol:acetonitrile (70:15:15, v/v/v). The proposed method provided linear responses within the concentration range 1.0-6.0 mu g mL(-1) for ciprofloxacin hydrochloride. Correlation coefficient (r) for the ciprofloxacin hydrochloride was 0.9994. The precision of the method was demonstrated using intra- and inter-day assay RSD% values which were less than 5% in all instances. No interference from any components of pharmaceutical dosage forms was observed.
Descrição
Palavras-chave
Column liquid chromatography, Ophthalmic solution, Fluoroquinolone, Ciprofloxacin
Idioma
Inglês
Como citar
Chromatographia. Wiesbaden: Vieweg, v. 69, p. 241-244, 2009.
