Detection of leptospires in bovine semen by polymerase chain reaction
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Coorientador
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Editor
Wiley
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Resumo
Objective: In view of the considerable importance of venereal transmission of bovine leptospirosis, the objective of the present study was to compare the polymerase chain reaction (PCR), culture/isolation and serology to detect leptospire infection in bovine semen. Design: Blood for serologic examination and semen for bacterial culture and PCR were collected from 20 bulls at artificial insemination centres in Brazil. Each animal was sampled twice for serology. Result: Forty-five percent (9/20) of the serum samples collected showed agglutinin titers to serovar hardjo in the first sample and 25% (5/20) had agglutinin titers to serovar hardjo in the second sample. Eighty percent (16/20) of semen samples were positive by PCR. Leptospires could not be isolated from any of the semen samples examined. Conclusion: Polymerase chain reaction can be a method of great potential for the detection of leptospires at artificial insemination centres.
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Palavras-chave
Cattle , Leptospirosis , PCR , Semen , bacterium antibody , animal , animal disease , artificial insemination , blood , Brazil , cattle , cattle disease , disease transmission , female , isolation and purification , Leptospira , leptospirosis , methodology , microbiology , polymerase chain reaction , species difference , sperm , Animals , Antibodies, Bacterial , Cattle Diseases , Female , Insemination, Artificial , Polymerase Chain Reaction , Species Specificity
Idioma
Inglês
Citação
Australian Veterinary Journal, v. 77, n. 1, p. 32-34, 1999.
