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Detection of leptospires in bovine semen by polymerase chain reaction

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dc.contributor.authorHeinemann, M. B.
dc.contributor.authorGarcia, J. F. [UNESP]
dc.contributor.authorNunes, C. M. [UNESP]
dc.contributor.authorMorais, Z. M.
dc.contributor.authorGregori, F.
dc.contributor.authorCortez, A.
dc.contributor.authorVasconcellos, S. A.
dc.contributor.authorVisintin, J. A.
dc.contributor.authorRichtzenhain, L. J.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-27T11:19:41Z
dc.date.available2014-05-27T11:19:41Z
dc.date.issued1999-01-01
dc.description.abstractObjective: In view of the considerable importance of venereal transmission of bovine leptospirosis, the objective of the present study was to compare the polymerase chain reaction (PCR), culture/isolation and serology to detect leptospire infection in bovine semen. Design: Blood for serologic examination and semen for bacterial culture and PCR were collected from 20 bulls at artificial insemination centres in Brazil. Each animal was sampled twice for serology. Result: Forty-five percent (9/20) of the serum samples collected showed agglutinin titers to serovar hardjo in the first sample and 25% (5/20) had agglutinin titers to serovar hardjo in the second sample. Eighty percent (16/20) of semen samples were positive by PCR. Leptospires could not be isolated from any of the semen samples examined. Conclusion: Polymerase chain reaction can be a method of great potential for the detection of leptospires at artificial insemination centres.en
dc.description.affiliationSão Paulo State University UNESP Campus Araçatuba, R. Clovis Pestana, 793, Araçatuba, SP, 16050-680
dc.description.affiliationFaculdade de Med. Vet. e Zootecnia University of São Paulo, Av. Prof. Dr. Orlando Marques Paiva, São Paulo, SP, 05508-000
dc.description.affiliationUnespSão Paulo State University UNESP Campus Araçatuba, R. Clovis Pestana, 793, Araçatuba, SP, 16050-680
dc.format.extent32-34
dc.identifierhttp://dx.doi.org/10.1111/j.1751-0813.1999.tb12422.x
dc.identifier.citationAustralian Veterinary Journal, v. 77, n. 1, p. 32-34, 1999.
dc.identifier.doi10.1111/j.1751-0813.1999.tb12422.x
dc.identifier.issn0005-0423
dc.identifier.scopus2-s2.0-0032610351
dc.identifier.urihttp://hdl.handle.net/11449/65692
dc.language.isoeng
dc.relation.ispartofAustralian Veterinary Journal
dc.relation.ispartofjcr0.860
dc.relation.ispartofsjr0,456
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectCattle
dc.subjectLeptospirosis
dc.subjectPCR
dc.subjectSemen
dc.subjectbacterium antibody
dc.subjectanimal
dc.subjectanimal disease
dc.subjectartificial insemination
dc.subjectblood
dc.subjectBrazil
dc.subjectcattle
dc.subjectcattle disease
dc.subjectdisease transmission
dc.subjectfemale
dc.subjectisolation and purification
dc.subjectLeptospira
dc.subjectleptospirosis
dc.subjectmethodology
dc.subjectmicrobiology
dc.subjectpolymerase chain reaction
dc.subjectspecies difference
dc.subjectsperm
dc.subjectAnimals
dc.subjectAntibodies, Bacterial
dc.subjectCattle Diseases
dc.subjectFemale
dc.subjectInsemination, Artificial
dc.subjectPolymerase Chain Reaction
dc.subjectSpecies Specificity
dc.titleDetection of leptospires in bovine semen by polymerase chain reactionen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dspace.entity.typePublication
unesp.author.lattes1892359871207408[3]
unesp.author.orcid0000-0002-5463-3845[3]

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