Publicação: Fractal dimension analysis: A new tool for analyzing colony-forming units
Carregando...
Data
Orientador
Coorientador
Pós-graduação
Curso de graduação
Título da Revista
ISSN da Revista
Título de Volume
Editor
Tipo
Artigo
Direito de acesso
Resumo
The gold standard for quantifying bacteria both in routine diagnostics and in research is plating followed by count of colony-forming units (CFU). But, manual CFU counting on plates is time-consuming and subjective. We evaluated fractal dimension as a new methodology for evaluating CFU. Twenty fragments of expanded polytetrafluoroethylene (ePTFE) synthetic vascular prosthesis and 20 silicone prostheses were embedded in bacterial suspensions and incubated. The prostheses were then sown in solid culture medium and incubated for 48 h. Petri dishes were photographed and analyzed by fractal dimension. There was correlation between the number of CFU in manual counting and the fractal dimension analysis (p = 0.0001). We demonstrated that fractal dimension is a useful method for microbiological analyses in researches. It makes CFU analysis easier and faster and can be used regardless of the culture medium. • Petri dishes with different bacterial colonies were photographed with a digital camera under natural light. • The images were binarized and analyzed with ImageJⓇ's “fractal dimension” tool. • Fractal dimension analysis showed to be a good tool for evaluating the amount of colony-forming unit.
Descrição
Palavras-chave
Colony-forming units counting (CFU), Computer-assisted, Image processing, Microbial colony count, Prostheses and implants
Idioma
Inglês
Como citar
MethodsX, v. 8.
