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dc.contributor.authorStorti, A. [UNESP]
dc.contributor.authorManzato, A. J. [UNESP]
dc.contributor.authorPizzolitto, Antonio Carlos [UNESP]
dc.contributor.authorPizzolitto, Elisabeth Loshchagin [UNESP]
dc.date.accessioned2014-06-24T19:55:13Z
dc.date.available2014-06-24T19:55:13Z
dc.date.issued2006
dc.identifierhttp://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/view/384
dc.identifier.citationRevista de Ciências Farmacêuticas Básica e Aplicada, v. 27, n. 3, 2006, p. 213-220.
dc.identifier.issn1808-4532
dc.identifier.issn2179-443X
dc.identifier.urihttp://hdl.handle.net/11449/108311
dc.description.abstractSemiquantitative (Maki) and quantitative (Brun- Buisson) culture techniques were employed in the diagnosis of catheter-related bloodstream infections (CRBSI) in patients who have a short-term central venous catheter (inserted for 30 days). The diagnosis of CRBSI was based on the results of semiquantitative and quantitative culture of material from the removed catheters. Catheter tips (118) from 100 patients were evaluated by both methods. Semiquantitative analysis revealed 34 catheters (28.8%) colonized by ≥15 colonyforming units (cfu), while quantitative cultures (34 catheters, 28.8%) showed the growth of ≥103 cfu/mL. Bacteremia was confirmed in four patients by isolating microorganisms of identical species from both catheters and blood samples. Using the semiquantitative culture technique on short-term central venous catheter tips, we have shown that with a cut-off level of ≥15 cfu, the technique had 100.0% sensitivity, specificity of 68.4%, 25.0% positive predictive value (PPV) and 100.0% negative predictive value (NPV), efficiency of 71.4% and a prevalence of 9.5%. The quantitative method, with a cut-off limit of ≥103 cfu/mL, gave identical values: the sensitivity was 100.0%, specificity 68.4%, positive predictive value (PPV) 25.0%, negative predictive value (NPV) 100.0%, efficiency 71.4% and prevalence 9.5%. We concluded that the semiquantitative and quantitative culture methods, evaluated in parallel, for the first time in Brazil, have similar sensitivity and specificity. Keywords: central venous catheter; semi-quantitative culture; quantitative culture; catheter-related bacteremia.en
dc.language.isoeng
dc.publisherUniversidade Estadual Paulista (UNESP)
dc.relation.ispartofRevista de Ciências Farmacêuticas Básica e Aplicada
dc.sourceRevista de Ciências Farmacêuticas Básica e Aplicada
dc.subjectcentral venous catheterpt
dc.subjectsemi-quantitative culturept
dc.subjectquantitative culturept
dc.subjectcatheter-related bacteremiapt
dc.subjectFarmacotécnicapt
dc.titleAssessment of central venous catheter-associated infections using semi-quantitative or quantitative culture methodsen
dc.typeArtigo
dcterms.licensehttp://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/about
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.description.affiliationDepartamento de Análises Clínicas, Faculdade de Ciências Farmacêuticas, Universidade Estadual Paulista, UNESP, Araraquara, SP, Brasil.
dc.description.affiliationDepartamento de Ciências de Computação e Estatística, Instituo de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista, UNESP, São Jose do Rio Preto, SP, Brasil
dc.description.affiliationUnespDepartamento de Análises Clínicas, Faculdade de Ciências Farmacêuticas, Universidade Estadual Paulista, UNESP, Araraquara, SP, Brasil.
dc.description.affiliationUnespDepartamento de Ciências de Computação e Estatística, Instituo de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista, UNESP, São Jose do Rio Preto, SP, Brasil
dc.rights.accessRightsAcesso aberto
dc.identifier.scopus2-s2.0-34948826558
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Ciências Farmacêuticas, Araraquarapt
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt
dc.identifier.fileISSN1808-4532-2006-27-3-213-220.pdf
dc.relation.ispartofsjr0,131
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