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dc.contributor.authorSilva Junior, Willer Ferreira da
dc.contributor.authorCano, Rodrigo
dc.contributor.authorTotola, Antonio Helvecio
dc.contributor.authorCarvalho, Lorendane Milena de
dc.contributor.authorCerri, Marcel Otavio [UNESP]
dc.contributor.authorReis Coimbra, Jane Sella dos
dc.contributor.authorPinto de Carvalho, Gleidson Giordano
dc.contributor.authorAparecida de Carvalho, Bruna Mara
dc.identifier.citationJournal Of Chromatography A. Amsterdam: Elsevier Science Bv, v. 1395, p. 16-22, 2015.
dc.description.abstractThe influence of temperature, pH and ionic strength on the adsorption of Immunoglobulin Y (IgY) in IDA-Cu2+ cryogel system was studied by batch equilibrium Measurements. The adsorptive equilibrium data were obtained at 17 and 27 degrees C, pH 5.0 and 6.5, and at ionic strength of 50 and 200 mmol L-1 NaCl. Langmuir, Freundlich and Langmuir-Freundlich models were fitted to equilibrium data, while the enthalpy of adsorption of IgY in IDA-Cu2+ cryogel system was calculated through Van't Hoff analysis. The binding of IgY on cryogel was stronger at 27 degrees C and lowest pH and ionic strength values, with apparent maximum adsorption capacity of 27 mg g(-1). The adsorption of protein in the resin was spontaneous in all analyzed cases. The results provide valuable information to enable the improvement of IgY purification processes. (C) 2015 Elsevier BY. All rights reserved.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.publisherElsevier B.V.
dc.relation.ispartofJournal Of Chromatography A
dc.sourceWeb of Science
dc.subjectImmunoglobulin Yen
dc.subjectAdsorption isothermsen
dc.subjectThermodynamic parametersen
dc.titleAdsorption of immunoglobulin Y in supermacroporous continuous cryogel with immobilized Cu2+ ionsen
dcterms.rightsHolderElsevier B.V.
dc.contributor.institutionUniv Fed Sao Joao del Rei
dc.contributor.institutionUniversidade Federal de Viçosa (UFV)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade Federal da Bahia (UFBA)
dc.contributor.institutionUniversidade Federal de Minas Gerais (UFMG)
dc.description.affiliationUniv Fed Sao Joao del Rei, Dept Chem Biotechnol &Bioproc Engn, BR-36420000 Ouro Branco, MG, Brazil
dc.description.affiliationUniv Fed Vicosa, Dept Vet, BR-36570000 Vicosa, MG, Brazil
dc.description.affiliationSao Paulo State Univ, Dept Bioproc &Biotechnol, BR-14801902 Araraquara, SP, Brazil
dc.description.affiliationUniv Fed Vicosa, Dept Food Technol, BR-36570000 Vicosa, MG, Brazil
dc.description.affiliationUniv Fed Bahia, Dept Anim Sci, BR-40110909 Salvador, BA, Brazil
dc.description.affiliationUniv Fed Minas Gerais, Inst Agr Sci, BR-39400401 Montes Claros, MG, Brazil
dc.description.affiliationUnespSao Paulo State Univ, Dept Bioproc &Biotechnol, BR-14801902 Araraquara, SP, Brazil
dc.rights.accessRightsAcesso restrito
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Ciências Farmacêuticas, Araraquarapt[5][6]
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