Pyrazinamide susceptibility testing in Mycobacterium tuberculosis using the fast resazurin microtiter assay plate
Nenhuma Miniatura disponível
Data
2016-11-01
Autores
Pina, R. Z.
Caleffi-Ferracioli, K. R.
Campanerut-Sa, R. A. Z.
Ghiraldi-Lopez, L. D.
Pavan, F. R. [UNESP]
Siqueira, V. L. D.
Scodro, R. B. L.
Cardoso, R. F.
Título da Revista
ISSN da Revista
Título de Volume
Editor
Int Union Against Tuberculosis Lung Disease (i U A T L D)
Resumo
SETTING: Department of Clinical Analysis and Biomedicine, State University of Maringa, Maringa, PR, Brazil. OBJECTIVE: To evaluate the performance of the resazurin microtiter assay (REMA) plate at pH 5.5 in detecting Mycobacterium tuberculosis susceptibility to pyrazinamide (PZA). DESIGN: The minimal inhibitory concentration (MIC) of PZA in M. tuberculosis H(37)Rv and M. bovis AN5 reference strains and in 34 clinical M. tuberculosis isolates (26 PZA-susceptible and eight PZA-resistant) was determined using REMA at pH 5.5 and compared to REMA at pH 6.0. RESULTS: REMA at pH 5.5 was helpful in discriminating PZA-susceptible from resistant M. tuberculosis isolates when <= 50 mu g/ml PZA was considered as the cut-off for PZA susceptibility. Furthermore, it provided results in 8 days. However, two PZA-resistant isolates failed to grow at pH 5.5. CONCLUSION: As the REMA method is rapid, inexpensive, easy to perform and read, it would be of great usefulness in low-income countries for detecting PZA-resistant M. tuberculosis. REMA at pH 5.6-5.9 should be evaluated on an extended panel of clinical M. tuberculosis isolates with a greater range of MIC values in different laboratories for a better understanding of its utility in differentiating PZA-resistant from PZA-susceptible isolates.
Descrição
Palavras-chave
tuberculosis, PZA resistance, REMA
Como citar
International Journal Of Tuberculosis And Lung Disease. Paris: Int Union Against Tuberculosis Lung Disease (i U A T L D), v. 20, n. 11, p. 1535-1538, 2016.