Microsatellite loci in the tiger shark and cross-species amplification using pyrosequencing technology
Carregando...
Data
2016-08-30
Autores
Mendes, Natalia J. [UNESP]
Cruz, Vanessa P. [UNESP]
Ashikaga, Fernando Y. [UNESP]
Camargo, Samia M. [UNESP]
Oliveira, Claudio [UNESP]
Piercy, Andrew N.
Burgess, George H.
Coelho, Rui
Santos, Miguel N.
Mendonca, Fernando F.
Título da Revista
ISSN da Revista
Título de Volume
Editor
Peerj Inc
Resumo
The tiger shark (Galeocerdo cuvier) has a global distribution in tropical and warm temperate seas, and it is caught in numerous fisheries worldwide, mainly as bycatch. It is currently assessed as near threatened by the International Union for Conservation of Nature (IUCN) Red List. In this study, we identified nine microsatellite loci through next generation sequencing (454 pyrosequencing) using 29 samples from the western Atlantic. The genetic diversity of these loci were assessed and revealed a total of 48 alleles ranging from 3 to 7 alleles per locus (average of 5.3 alleles). Cross-species amplification was successful at most loci for other species such as Carcharhinus longimanus, C. acronotus and Alopias superciliosus. Given the potential applicability of genetic markers for biological conservation, these data may contribute to the population assessment of this and other species of sharks worldwide.
Descrição
Palavras-chave
High-throughput sequencing, Galeocerdo cuvier, Shark, Microsatellites, Population structure
Como citar
Peerj. London: Peerj Inc, v. 4, 8 p., 2016.